COPE - Antibodies - The Human Protein Atlas

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COPE

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA041605

Antibody HPA043576

Antibody CAB034345

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA041605

HPA043576

sc-133195

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA041605, RRID:AB_2677578

Atlas Antibodies Cat#HPA043576, RRID:AB_2678563

Santa Cruz Biotechnology Cat#sc-133195, RRID:AB_2081323

VALIDATION SUMMARY

Antibody HPA041605

Antibody HPA043576

Antibody CAB034345

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA041605

Antibody HPA043576

Antibody CAB034345

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA043576.

Validated

Antibody staining overlaps with antibody HPA041605.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus.

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

Antibody dilution

1:44

1:8

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA041605

Antibody HPA043576

Antibody CAB034345

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA043576 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA041605 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA043576 across 71 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA041605 across 71 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 68 cell types

RNA: detected in 37 tissues
Protein: detected in 56 cell types

RNA: detected in 37 tissues
Protein: detected in 51 cell types

Retrieval

HIER pH6

Antibody dilution

1:65

1:125

1:3000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA041605

Antibody HPA043576

Antibody CAB034345

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

36.9, 34.5, 28.8

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA041605

Antibody HPA043576

Antibody CAB034345

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:12000

1:500

ANTIGEN INFORMATION

Antibody HPA041605

Antibody HPA043576

Antibody CAB034345

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

DATLTQLATAWVSLATGGEKLQDAYYIFQEMADKCSPTLLLLNGQAACHM
AQGRWEAAEGLLQEALDKDSGYPETLVNL

GSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPERDVERDVFLYRAYL
AQRKFGVVLDEIKPSSAPELQAVRMFADYL

Matching transcripts

COPE-001 - ENSP00000262812 [100%]
COPE-002 - ENSP00000345674 [100%]
COPE-004 - ENSP00000469035 [100%]
COPE-201 - ENSP00000483420 [100%]

COPE-001 - ENSP00000262812 [100%]
COPE-002 - ENSP00000345674 [100%]
COPE-004 - ENSP00000469035 [100%]
COPE-005 - ENSP00000343134 [100%]
COPE-201 - ENSP00000483420 [100%]

Other gene match

ANTIGEN VIEW

COPE-001	COPE-002	COPE-004	COPE-005	COPE-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.