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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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ARHGAP26
  • TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene namei

Gene name according to HGNC.

ARHGAP26

Gene descriptioni

Gene description according to HGNC.

Rho GTPase activating protein 26

Protein classi

Protein class the gene product belongs to according to selected gene lists. List of protein classes.

Cancer-related genes
Disease related genes
Predicted intracellular proteins
Predicted membrane proteins

Predicted localizationi

Protein localization predicted from several bioinformatic algorithms.

Read more

Intracellular,Membrane

Number of transcriptsi

Number of protein-coding transcribed from this gene as defined by Ensembl.

9
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA categoryi

RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue/cell line enriched, group enriched, tissue/cell line enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for The Cancer Genome Atlas (TCGA) data from cancer tissues and internally generated Human Protein Atlas (HPA) data from normal tissues and cell lines.

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (Karpas-707)
HPA (normal tissue):Mixed

Protein evidencei

Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.

Evidence at protein level

Protein expression
normal tissuei

A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.

"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.

Read more

Cytoplasmic and membrane expression in most tissues.

ANTIBODY IHC RELIABILITY

Data reliability
descriptioni

Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.

Antibody staining mainly consistent with RNA expression data.

Reliability scorei

Reliability score (score description), divided into Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between the staining pattern of one antibody or several antibodies with RNA-seq data and available gene/protein characterization data.

Read more

Approved based on 2 antibodies.
HPA035106 , HPA035107
SHOW MORE

PROGNOSTIC SUMMARYi

Kaplan-Meier plots for all cancers where high expression of this gene has significant (p<0.001) association with patient survival are shown in this summary. Whether the prognosis is favourable or unfavourable is indicated in brackets. Each Kaplan-Meier plot is clickable and redirects to a detailed page that includes individual expression and survival data for patients with the selected cancer.

Prognostic marker in urothelial cancer (favourable), endometrial cancer (favourable), cervical cancer (favourable), breast cancer (favourable) and colorectal cancer (favourable).
Urothelial cancer p<0.001
Endometrial cancer p<0.001
Cervical cancer p<0.001
Breast cancer p<0.001
Colorectal cancer p<0.001
Show all

RNA EXPRESSION OVERVIEWi

RNA expression overview shows RNA-seq data from The Cancer Genome Atlas (TCGA).

TCGA dataseti
RNA-seq data in 17 cancer types are reported as median FPKM (number Fragments Per Kilobase of exon per Million reads), generated by the The Cancer Genome Atlas (TCGA). RNA cancer tissue category is calculated based on mRNA expression levels across all 17 cancer tissues and include: cancer tissue enriched, cancer group enriched, cancer tissue enhanced, expressed in all, mixed and not detected. To access cancer specific RNA and prognostic data, click on the cancer name.

Read more
RNA cancer category: Expressed in all

PROTEIN EXPRESSIONi

Antibody staining in 20 different cancers is summarized by a selection of four standard cancer tissue samples representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional fifth image can be added as a complement. The assay and annotation is described here. Note that samples used for immunohistochemistry by the Human Protein Atlas do not correspond to samples in the TCGA dataset.

For histological reference, visit the histological dictionary.

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARYi

For each cancer, the fraction of samples with protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the left). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The mouse-over function displays additional data for the features in the staining summary view.

Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.

HPA035106
HPA035107

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summaryi

A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.

Most cancer tissues exhibited weak to moderate cytoplasmic immunoreactivity, with additional membranous staining in prostate and thyroid cancers. Squamous cell carcinomas, lymphomas, testicular, urothelial and stomach cancers were mostly negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summaryi

A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.

Colorectal cancers showed moderate luminal membranous positivity. Few cases of duct carcinomas of breast, ovarian, testicular and urothelial exhibited weak moderate membranous positivity. Smooth muscle cells were distinctly stained. Remaining cancer tissues were mostly negative.

GENE INFORMATIONi

Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.

Gene name

ARHGAP26 (HGNC Symbol)

Synonyms

GRAF, KIAA0621, OPHN1L, OPHN1L1

Description

Rho GTPase activating protein 26 (HGNC Symbol)

Entrez gene summary

Interaction of a cell with the extracellular matrix triggers integrin cell surface receptors to begin signaling cascades that regulate the organization of the actin-cytoskeleton. One of the proteins involved in these cascades is focal adhesion kinase. The protein encoded by this gene is a GTPase activating protein that binds to focal adhesion kinase and mediates the activity of the GTP binding proteins RhoA and Cdc42. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Chromosome

5

Cytoband

q31.3

Chromosome location (bp)

142770384 - 143229011

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000145819 (version 83.38)

Entrez gene

23092

UniProt

Q9UNA1 (UniProt - Evidence at protein level)

neXtProt

NX_Q9UNA1

Antibodypedia

ARHGAP26 antibodies

PROTEIN BROWSERi

The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.

At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.

Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).

If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.

Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.

ARHGAP26-001
ARHGAP26-008
ARHGAP26-009
ARHGAP26-012
ARHGAP26-013
ARHGAP26-014
ARHGAP26-015
ARHGAP26-020
ARHGAP26-021

PROTEIN INFORMATIONi

The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.

The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.

The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.

The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ARHGAP26-001
ENSP00000274498
ENST00000274498
Q9UNA1 [Direct mapping]
Rho GTPase-activating protein 26
Show all
   Phobius predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0005515 [protein binding]
GO:0005543 [phospholipid binding]
GO:0005575 [cellular_component]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005925 [focal adhesion]
GO:0007009 [plasma membrane organization]
GO:0007165 [signal transduction]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007399 [nervous system development]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
814 aa
92.2 kDa
No 0
ARHGAP26-008
ENSP00000367243
ENST00000378004
Q9UNA1 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0005515 [protein binding]
GO:0005543 [phospholipid binding]
GO:0005575 [cellular_component]
GO:0005829 [cytosol]
GO:0005856 [cytoskeleton]
GO:0005925 [focal adhesion]
GO:0007009 [plasma membrane organization]
GO:0007165 [signal transduction]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007399 [nervous system development]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
759 aa
86.2 kDa
No 0
ARHGAP26-009
ENSP00000392186
ENST00000443045
H7BZZ0 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0007165 [signal transduction]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
97 aa
10.9 kDa
No 0
ARHGAP26-012
ENSP00000389137
ENST00000421521
H7BZE1 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0005515 [protein binding]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
154 aa
16.2 kDa
No 1
ARHGAP26-013
ENSP00000403388
ENST00000425417
H7C205 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0005515 [protein binding]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
98 aa
10.1 kDa
No 0
ARHGAP26-014
ENSP00000367252
ENST00000378013
F8W8N5 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
166 aa
19.5 kDa
No 0
ARHGAP26-015
ENSP00000411571
ENST00000451259
C9J6V4 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
91 aa
10.2 kDa
No 0
ARHGAP26-020
ENSP00000416889
ENST00000418236
H0Y835 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0005515 [protein binding]
GO:0007165 [signal transduction]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
294 aa
32.8 kDa
No 0
ARHGAP26-021
ENSP00000393276
ENST00000443674
H0Y4P9 [Direct mapping]
Rho GTPase-activating protein 26
Show all
Predicted intracellular proteins
Cancer-related genes
   COSMIC somatic mutations in cancer genes
   COSMIC Splicing Mutations
   COSMIC Somatic Mutations
   COSMIC Frameshift Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005096 [GTPase activator activity]
GO:0005515 [protein binding]
GO:0007165 [signal transduction]
GO:0030036 [actin cytoskeleton organization]
GO:0043547 [positive regulation of GTPase activity]
GO:0051056 [regulation of small GTPase mediated signal transduction]
Show all
388 aa
42.9 kDa
No 0

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.