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ANTIBODY INFORMATION
 
 

Antibody HPA010928

  

Antibody CAB009313

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA010928   sc-13979  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 5   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA010928, RRID:AB_1857425   Santa Cruz Biotechnology Cat#sc-13979, RRID:AB_2185502  

VALIDATION SUMMARY
 
 

Antibody HPA010928

  

Antibody CAB009313

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010928

  

Antibody CAB009313

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human lung shows distinct cytoplasmic positivity in Type II pneumocytes.   Immunohistochemical staining of human lung shows cytoplasmic positivity in type II alveolar cells.  

Expression

 RNA: detected in 14 tissues
Protein: detected in 2 cell types		   RNA: detected in 14 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:175  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010928

  

Antibody CAB009313

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 21, 20.3, 15.3, 13.1   21, 20.3, 17.7, 17.4, 16.2, 15.3, 13.1  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA010928

  

Antibody CAB009313

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA010928

  

Antibody CAB009313

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 109    

Antigen sequence

 
PEAQQRLALSEHLVTTATFSIGSTGLVVYDYQQLLIAYKPAPGTCCYIMK
IAPESIPSLEALTRKVHNFQAKPAVPTSKLGQAEGRDAGSAPSGGDPAFL
GMAVSTLCG
  

											 
																				

																		

										
											
Matching transcripts

												
																					SFTPC-003 - ENSP00000430410 [100%] 
SFTPC-001 - ENSP00000316152 [95%] 
SFTPC-007 - ENSP00000429552 [95%] 
SFTPC-008 - ENSP00000429619 [94%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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