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RGS19
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA070250

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA070250  

Host species

Rabbit  

Clonalityi

The antibodies are designated mAB for monoclonal and pAb for polyclonal.

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene matchi

Genes, excluding the target gene, to which the antigen sequence of the antibody is >80% identical.

 

Released in versioni

The release of the Human Protein Atlas in which the antibody was first published.

16  

Referencesi

References to publications in which the antibody has been used.

 

Proper citation

Atlas Antibodies Cat#HPA070250, RRID:AB_2686241  

VALIDATION SUMMARYi

Read more

 
 

Antibody HPA070250

 
Immunocytochemistryi
Immunocytochemistry is used to validate the antibody staining and for assessing and validating the protein expression pattern in selected human cell lines.

Read more

Approved  
Western bloti
A Western blot analysis is performed on a panel of human tissues and cell lines to evaluate antibody specificity. For antibodies with uncertain result a revalidation using an over-expression lysate is performed.

Read more

Uncertain  
Protein arrayi
A protein microarray containing 384 different antigens including the antibody target is used for analysis of antibody specificity.

Read more

Approved  

IMMUNOCYTOCHEMISTRYi

Immunocytochemistry is used to validate the antibody staining and for assessing and validating the protein expression pattern in selected human cell lines.

Read more

 
 

Antibody HPA070250

 

Standard validationi

For each antibody, the observed staining in the different cell lines is assigned a validation score based on concordance with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database. The validation scores for up to three cell lines are merged into one of the main categories; Supported, Approved, or Uncertain to represent the overall antibody staining in all analyzed cell lines.

Read more

Approved  

 

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli & vesicles.  

Antibody dilution

1:42  

Literature conformityi

The immunostaining patterns is compared for consistency with available experimental gene/protein characterization data in the UniProtKB/Swiss-Prot database and other experimental evidence for location described in scientific literature.

Read more

The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOTi

A Western blot analysis is performed on a panel of human tissues and cell lines to evaluate antibody specificity. For antibodies with unreliable result a revalidation using an over-expression lysate is performed.

Read more

 
 

Antibody HPA070250

 

Standard validationi

Western Blot is used for quality control of the polyclonal antibodies generated in the project. After purification, the antibodies are used to detect bands in a setup of lysate and different tissues. The result is then scored Supported, Approved, or Uncertain.

Read more

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.
 

 

Figure description

 

Target mass (kDa)

24.6  

Antibody dilution

1:140  

PROTEIN ARRAYi

A protein microarray containing 384 different antigens including the antibody target is used for analysis of antibody specificity.

Read more

 
 

Antibody HPA070250

 

Standard validationi

A protein array containing 384 different antigens including the antibody target is used to analyse antibody specificity. Depending on the array interaction profile the antibody is scored as Supported, Approved, or Uncertain.

Read more

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:1700  

ANTIGEN INFORMATION

 
 

Antibody HPA070250

 

Antigen

Recombinant protein fragment  

Length (aa)

43  

Antigen sequence

PHEAEKQITGPEEADRPPSMSSHDTASPAAPSRNPCCLCWCCC
 

Matching transcripts

RGS19-001 - ENSP00000333194 [100%]
RGS19-201 - ENSP00000378483 [100%]
 

Other gene matchi

Genes, excluding the target gene, to which the antigen sequence of the antibody is >80% identical.

 
ANTIGEN VIEW
RGS19-001
RGS19-201
 

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