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SMIM11A

ANTIBODY INFORMATION
 
 

Antibody HPA051321

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA051321  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 SMIM11B - ENSG00000273590 [100%]
  

Released in version

 11  

References

  

Proper citation

 Atlas Antibodies Cat#HPA051321, RRID:AB_2681440  

VALIDATION SUMMARY
 
 

Antibody HPA051321

  
Immunocytochemistry

Approved  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051321

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to focal adhesion sites.  

Antibody dilution

 1:60  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051321

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 10 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:30  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051321

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

 

Figure description

  

Target mass (kDa)

 6.9  

Antibody dilution

 1:300  

PROTEIN ARRAY
 
 

Antibody HPA051321

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2400  

ANTIGEN INFORMATION
 
 

Antibody HPA051321

  

Antigen

 Recombinant protein fragment  

Length (aa)

 26  

Antigen sequence

 
YQRKRLEAKQQKLEAERKKQSEKKDN
 

Matching transcripts

 SMIM11A-003 - ENSP00000382231 [100%]
SMIM11A-004 - ENSP00000382234 [100%]
 

Other gene match

 SMIM11B - ENSG00000273590 [100%]
  
ANTIGEN VIEW
SMIM11A-003
SMIM11A-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.