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Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of cytoplasmic bodies in human cell line U-2 OS (HPA013202)
Scale bar represents 10µm
Cytoplasmic bodies
Cytoplasmic body is a collective term for distinct foci inside the cytoplasm including processing bodies (P-bodies) or stress granules. P-bodies have important tasks in mRNA turnover, including mRNA decay and silencing.
Immunofluorescent staining
The staining of cytoplasmic bodies appears as distinct round spots in the cytoplasm and can easily be mistaken for vesicles. However, it is usually possible to distinguish them by their size and number, as cytoplasmic bodies are slightly larger than vesicles and occur in lower numbers.
