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Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, cells in the molecular layer and cells in the granular layer.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in cells in molecular layer, cells in granular layer
Expression
RNA: detected in 2 tissues Protein: detected in 13 cell types
RNA: detected in 2 tissues Protein: detected in 18 cell types
Retrieval
HIER pH6
None
Antibody dilution
1:500
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035876
Antibody CAB002681
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401075)
Target mass (kDa)
14.3, 12.9
14.3, 12.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA035876
Antibody CAB002681
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.