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GCN1

ANTIBODY INFORMATION
 
 

Antibody HPA018799

  

Antibody HPA019648

  

Antibody HPA024367

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA018799   HPA019648   HPA024367  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   5   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA018799, RRID:AB_1849565   Atlas Antibodies Cat#HPA019648, RRID:AB_1849567   Atlas Antibodies Cat#HPA024367, RRID:AB_1849569  

VALIDATION SUMMARY
 
 

Antibody HPA018799

  

Antibody HPA019648

  

Antibody HPA024367

  
Immunocytochemistry


Validated
  

Validated

Validated
    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain  

Validated
   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018799

  

Antibody HPA019648

  

Antibody HPA024367

  

Formal validation: Genetic

  
Validated

Significant downregulation > 25% by both siRNA:s.		    

 
   

Figure description

   Cytoplasmic region of segmented cells in 10x-images    

Antibody dilution

   1:35    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA019648.		  
Validated

Antibody staining overlaps with antibody HPA018799.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.    

Antibody dilution

 1:20   1:20    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018799

  

Antibody HPA019648

  

Antibody HPA024367

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human pancreas shows cytoplasmic positivity in exocrine glandular cells and islet cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:20   1:70   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018799

  

Antibody HPA019648

  

Antibody HPA024367

  

Formal validation: Genetic

  
Validated

Downregulation visible in both siRNA lanes		    

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

   292.7    

Loading control

   Total protein image    

Antibody dilution

   1:78    
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 292.7   292.7   292.7  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA018799

  

Antibody HPA019648

  

Antibody HPA024367

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA018799

  

Antibody HPA019648

  

Antibody HPA024367

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 128   137   131  

Antigen sequence

 
EALVTDAGEVTEAGKAYVPPRVLQEALCVISGVPGLKGDVTDTEQLAQEM
LIISHHPSLVAVQSGLWPALLARMKIDPEAFITRHLDQIIPRMTTQSPLN
QSSMNAMGSLSVLSPDRVLPQLISTITA
  
ALKEKLGTPDEQLEMANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMR
QAAAIILNIYCSRSKADYTSHLRSLVSGLIRLFNDSSPVVLEESWDALNA
ITKKLDAGNQLALIEELHKEIRLIGNESKGEHVPGFC
  
LLPLLIQTVEKAASQSTQVPTITEGVAAALLLLKLSVADSQAEAKLSSFW
QLIVDEKKQVFTSEKFLVMASEDALCTVLHLTERLFLDHPHRLTGNKVQQ
YHRALVAVLLSRTWHVRRQAQQTVRKLLSSL
 

Matching transcripts

 GCN1-001 - ENSP00000300648 [100%]
  GCN1-001 - ENSP00000300648 [100%]
  GCN1-001 - ENSP00000300648 [100%]
 

Other gene match

      
ANTIGEN VIEW
GCN1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.