We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm & cytosol.
Antibody dilution
1:109
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA064431
Antibody CAB009189
Standard validation
Supported
Figure description
Immunohistochemical staining of human lymph node shows strong nuclear and cytoplasmic positivity in subset of non-germinal center cells.
Expression
RNA: detected in 35 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
Antibody dilution
1:20
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA064431
Antibody CAB009189
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
165.9, 162.2, 160.9, 157.3, 154.9
165.9, 162.2, 160.9, 157.3, 154.9, 4
Antibody dilution
1:360
1:500
PROTEIN ARRAY
Antibody HPA064431
Antibody CAB009189
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
