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SLC35F2

ANTIBODY INFORMATION
 
 

Antibody HPA048185

  

Antibody HPA050695

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048185   HPA050695  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA048185, RRID:AB_2680304   Atlas Antibodies Cat#HPA050695, RRID:AB_2681217  

VALIDATION SUMMARY
 
 

Antibody HPA048185

  

Antibody HPA050695

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA048185

  

Antibody HPA050695

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

   1:28  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048185

  

Antibody HPA050695

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.    

Expression

 RNA: detected in 34 tissues
Protein: detected in 35 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:20    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA048185

  

Antibody HPA050695

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 41.2, 36.3   41.2, 39.2  

Antibody dilution

 1:240   1:140  

PROTEIN ARRAY
 
 

Antibody HPA048185

  

Antibody HPA050695

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1900   1:1100  

ANTIGEN INFORMATION
 
 

Antibody HPA048185

  

Antibody HPA050695

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 33   38  

Antigen sequence

 
AEPAESSVPPVTSIGIDNLGLKLEENLQETHSA
  
MEADSPAGPGAPEPLAEGAAAEFSSLLRRIKGKLFTWN
 

Matching transcripts

 SLC35F2-002 - ENSP00000436785 [100%]
SLC35F2-004 - ENSP00000364834 [100%]
  SLC35F2-001 - ENSP00000434307 [100%]
SLC35F2-002 - ENSP00000436785 [100%]
 

Other gene match

    
ANTIGEN VIEW
SLC35F2-001
SLC35F2-002
SLC35F2-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.