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Antibody staining overlaps with antibody HPA051576.
Validated
Antibody staining overlaps with antibody HPA022120.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & plasma membrane.
Antibody dilution
1:20
1:22
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA022120
Antibody HPA051576
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:65
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA022120
Antibody HPA051576
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
165.7, 131.8
165.7, 131.8
Antibody dilution
1:250
1:110
PROTEIN ARRAY
Antibody HPA022120
Antibody HPA051576
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
