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PNO1

ANTIBODY INFORMATION
 
 

Antibody HPA056928

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA056928  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

Proper citation

  

VALIDATION SUMMARY
 
 

Antibody HPA056928

  
Immunocytochemistry

Supported  
Western blot

Supported  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA056928

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nucleoli.  

Antibody dilution

 1:35  

Literature conformity

 The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA056928

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 27.9  

Antibody dilution

 1:120  

PROTEIN ARRAY
 
 

Antibody HPA056928

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:15000  

ANTIGEN INFORMATION
 
 

Antibody HPA056928

  

Antigen

 Recombinant protein fragment  

Length (aa)

 43  

Antigen sequence

 
HILGSFQNIKMARTAICNLILGNPPSKVYGNIRAVASRSADRF
 

Matching transcripts

 PNO1-001 - ENSP00000263657 [100%]
 

Other gene match

  
ANTIGEN VIEW
PNO1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.