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CBX3

ANTIBODY INFORMATION
 
 

Antibody HPA004902

  

Antibody CAB001973

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA004902   05-690  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

    

Released in version

 4   1  

References

 1    

Proper citation

 Atlas Antibodies Cat#HPA004902, RRID:AB_1850635    

VALIDATION SUMMARY
 
 

Antibody HPA004902

  

Antibody CAB001973

  
Immunocytochemistry

Approved    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004902

  

Antibody CAB001973

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear bodies.    

Antibody dilution

 1:29    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004902

  

Antibody CAB001973

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows distinct nuclear positivity in glandular cells.   Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:30   1:15  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004902

  

Antibody CAB001973

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 20.8   20.8, 12.4, 11.9, 7.2  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004902

  

Antibody CAB001973

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA004902

  

Antibody CAB001973

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 110    

Antigen sequence

 
RVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDG
TKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMF
LMKWKDSDEA
  

											 
																				

																		

										
											
Matching transcripts

												
																					CBX3-001 - ENSP00000336687 [100%] 
CBX3-002 - ENSP00000379670 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

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RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA004902

												
										 
																				
											
											
											
Antibody CAB001973

												
										 
																		

																	

										
											
1.

												
																						Somatostatin derivative (smsDX) targets cellular metabolism in prostate cancer cells after androgen deprivation therapy

	Yan L et al

	PLoS One 2013;8(2):e55790

			
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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