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ANTIBODY INFORMATION
 
 

Antibody HPA049900

  

Antibody HPA058396

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049900   HPA058396  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA049900, RRID:AB_2680942   Atlas Antibodies Cat#HPA058396, RRID:AB_2683704  

VALIDATION SUMMARY
 
 

Antibody HPA049900

  

Antibody HPA058396

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA049900

  

Antibody HPA058396

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA058396.		  
Validated

Antibody staining overlaps with antibody HPA049900.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.  

Antibody dilution

 1:30   1:26  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049900

  

Antibody HPA058396

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.    

Expression

 RNA: detected in 31 tissues
Protein: detected in 4 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:40    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA049900

  

Antibody HPA058396

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 53.4, 51.6   53.4, 51.6, 49.1  

Antibody dilution

 1:150   1:90  

PROTEIN ARRAY
 
 

Antibody HPA049900

  

Antibody HPA058396

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1200   1:900  

ANTIGEN INFORMATION
 
 

Antibody HPA049900

  

Antibody HPA058396

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 51   66  

Antigen sequence

 
IAGVFPQKFDGDSAYVGMSDGNPELLSTSQTYNGQSENNEDYEIPPITPP
N
  
HEASYHSLCHGLTPNGLLPAYSYQAMDLPAIMVSNMLAQDSHLLSGQLPT
IQEMVHSEVAAYDSGR
 

Matching transcripts

 TOX2-001 - ENSP00000350849 [100%]
TOX2-003 - ENSP00000344724 [86%]
  TOX2-001 - ENSP00000350849 [100%]
TOX2-002 - ENSP00000362090 [100%]
TOX2-003 - ENSP00000344724 [100%]
TOX2-006 - ENSP00000390278 [100%]
 

Other gene match

    
ANTIGEN VIEW
TOX2-001
TOX2-002
TOX2-003
TOX2-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.