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Immunofluorescent staining of human cell line A-431 shows localization to nucleus.
Antibody dilution
1:25
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038885
Antibody CAB009370
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human placenta shows nuclear positivity in decidual cells.
Immunohistochemical staining of human placenta shows distinct nuclear and cytoplasmic positivity in trophoblastic cells.
Expression
RNA: detected in 36 tissues Protein: detected in 46 cell types
RNA: detected in 36 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038885
Antibody CAB009370
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil