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CXCR5

ANTIBODY INFORMATION
 
 

Antibody HPA042432

  

Antibody CAB026149

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA042432   MAB190  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042432, RRID:AB_2677995    

VALIDATION SUMMARY
 
 

Antibody HPA042432

  

Antibody CAB026149

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042432

  

Antibody CAB026149

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.   Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra.  

Expression

 RNA: detected in 14 tissues
Protein: detected in 57 cell types		   RNA: detected in 14 tissues
Protein: detected in 40 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1500   1:2200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042432

  

Antibody CAB026149

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 42   42  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA042432

  

Antibody CAB026149

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:30000    

ANTIGEN INFORMATION
 
 

Antibody HPA042432

  

Antibody CAB026149

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 53    

Antigen sequence

 
MNYPLTLEMDLENLEDLFWELDRLDNYNDTSLVENHLCPATEGPLMASFK
AVF
  

											 
																				

																		

										
											
Matching transcripts

												
																					CXCR5-001 - ENSP00000292174 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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