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Immunohistochemical staining of human cerebral cortex shows moderate nuclear membranous positivity in neuronal cells.
Immunohistochemical staining of human uterus, pre-menopause shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human stomach, lower shows moderate nuclear positivity in glandular cells.
Expression
RNA: detected in 32 tissues Protein: detected in 17 cell types
RNA: detected in 32 tissues Protein: detected in 15 cell types
RNA: detected in 32 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:25
1:200
1:200
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055778
Antibody HPA058523
Antibody CAB025157
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
95.3, 74
95.3, 74
95.3, 74
Antibody dilution
1:110
1:320
1:500
PROTEIN ARRAY
Antibody HPA055778
Antibody HPA058523
Antibody CAB025157
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
