We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA015600 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA013392 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA015600 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA013392 across 73 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human cerebral cortex shows distinct neuropil positivity.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells and glial cells.
Expression
RNA: detected in 13 tissues Protein: detected in 17 cell types
RNA: detected in 13 tissues Protein: detected in 16 cell types
RNA: detected in 13 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:250
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA013392
Antibody HPA015600
Antibody CAB004417
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400750)
Target mass (kDa)
28.8, 24.8
28.8, 24.8
28.8, 24.8
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA013392
Antibody HPA015600
Antibody CAB004417
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.