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SF3A3

ANTIBODY INFORMATION
 
 

Antibody HPA032054

  

Antibody HPA032055

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA032054   HPA032055  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA032054, RRID:AB_10602184   Atlas Antibodies Cat#HPA032055, RRID:AB_2674130  

VALIDATION SUMMARY
 
 

Antibody HPA032054

  

Antibody HPA032055

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA032054

  

Antibody HPA032055

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:79    

Literature conformity

 The subcellular location is supported by literature.    
 

Validation: Genetic

 Supported

Significant downregulation 10-25% by one/two siRNA:s		    

   

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:79    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA032054

  

Antibody HPA032055

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 80 cell types		   RNA: detected in 37 tissues
Protein: detected in 74 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:35  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA032054

  

Antibody HPA032055

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.8   58.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA032054

  

Antibody HPA032055

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA032054

  

Antibody HPA032055

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 84   82  

Antigen sequence

 
TKKSTLRDQINSDHRTRAMQDRYMEVSGNLRDLYDDKDGLRKEELNAISG
PNEFAEFYNRLKQIKEFHRKHPNEICVPMSVEFE
  
LGWDGKPIPYWLYKLHGLNINYNCEICGNYTYRGPKAFQRHFAEWRHAHG
MRCLGIPNTAHFANVTQIEDAVSLWAKLKLQK
 

Matching transcripts

 SF3A3-001 - ENSP00000362110 [100%]
  SF3A3-001 - ENSP00000362110 [100%]
 

Other gene match

    
ANTIGEN VIEW
SF3A3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.