Fields »
ABOUT
HELP
BLOG
CD47

ANTIBODY INFORMATION
 
 

Antibody HPA044659

  

Antibody CAB016055

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA044659   sc-12730  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA044659, RRID:AB_2679040   Santa Cruz Biotechnology Cat#sc-12730, RRID:AB_627086  

VALIDATION SUMMARY
 
 

Antibody HPA044659

  

Antibody CAB016055

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044659

  

Antibody CAB016055

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, the Golgi apparatus & vesicles.  

Antibody dilution

   1:40  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044659

  

Antibody CAB016055

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.   Immunohistochemical staining of human urinary bladder shows moderate membranous positivity in urothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 36 cell types		   RNA: detected in 37 tissues
Protein: detected in 31 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:25  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044659

  

Antibody CAB016055

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 35.2, 33.2   35.2, 33.2, 11.4, 9.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA044659

  

Antibody CAB016055

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA044659

  

Antibody CAB016055

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 118    

Antigen sequence

 
AQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYT
FDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVT
ELTREGETIIELKYRVVS
  

											 
																				

																		

										
											
Matching transcripts

												
																					CD47-002 - ENSP00000347512 [100%] 
CD47-004 - ENSP00000355361 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD47-002
CD47-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.