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BTBD18

ANTIBODY INFORMATION
 
 

Antibody HPA049325

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA049325  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 10  

References

  

Proper citation

 Atlas Antibodies Cat#HPA049325, RRID:AB_2680713  

VALIDATION SUMMARY
 
 

Antibody HPA049325

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA049325

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 40 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:450  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA049325

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 77.9  

Antibody dilution

 1:800  

PROTEIN ARRAY
 
 

Antibody HPA049325

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6400  

ANTIGEN INFORMATION
 
 

Antibody HPA049325

  

Antigen

 Recombinant protein fragment  

Length (aa)

 96  

Antigen sequence

 
MPSEVSEVLSVGGRWTPDLEITSSQPLDGQEDKLLHVSSLDTPQRSYGDL
SPPCSNWVETGLEVSLTTDELLYPSPKAGKEVSGHSELLGSLPASS
 

Matching transcripts

 BTBD18-001 - ENSP00000397020 [100%]
BTBD18-201 - ENSP00000394472 [100%]
 

Other gene match

  
ANTIGEN VIEW
BTBD18-001
BTBD18-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.