PSMB9 - Antibodies - The Human Protein Atlas

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PSMB9

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA042818

Antibody HPA053280

Antibody CAB015180

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA042818

HPA053280

sc-28809

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA042818, RRID:AB_2678186

Atlas Antibodies Cat#HPA053280, RRID:AB_2682098

Santa Cruz Biotechnology Cat#sc-28809, RRID:AB_2172368

VALIDATION SUMMARY

Antibody HPA042818

Antibody HPA053280

Antibody CAB015180

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA042818

Antibody HPA053280

Antibody CAB015180

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

Antibody dilution

1:200

1:125

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA042818

Antibody HPA053280

Antibody CAB015180

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows weak nuclear positivity in germinal center cells.

Immunohistochemical staining of human lymph node shows moderate cytoplasmic and nuclear positivity in non-germinal and germinal center cells.

Expression

RNA: detected in 35 tissues
Protein: detected in 28 cell types

RNA: detected in 35 tissues
Protein: detected in 50 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:575

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA042818

Antibody HPA053280

Antibody CAB015180

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

23.3, 21, 13.9

Antibody dilution

1:250

1:90

1:500

PROTEIN ARRAY

Antibody HPA042818

Antibody HPA053280

Antibody CAB015180

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:750

ANTIGEN INFORMATION

Antibody HPA042818

Antibody HPA053280

Antibody CAB015180

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

GIELEEPPLVLAAANVVRNISYKYREDLSAHLMVAGWDQREGG

GVVMGSDSRVSAGEAVVNRVFDKLSPLHERIYCALSGS

Matching transcripts

PSMB9-001 - ENSP00000363993 [100%]
PSMB9-005 - ENSP00000378739 [100%]
PSMB9-006 - ENSP00000394363 [100%]

Other gene match

ANTIGEN VIEW

PSMB9-001	PSMB9-005	PSMB9-006

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.