Cell lines


Name

Description of cell line

Original reference

Origin

Sex & Age 

Source

Cellosaurus (ExPaSy)

Brain

AF22 iPS derived Neuroepithelia stem (NES) cell line Falk et al., PLoS One 7(1) (2012) Skin Female, 36 NA
U-87 MG* Glioblastoma, astrocytoma cell line Ponten J., Macintyre EH., Acta Pathol. Microbiol. Scand. 74: 465-486 (1968) Brain Male, 44 LGC/ATCC CVCL_0022
Cancer cell line
U-251 MG Glioblastoma cell line Westermark B., Int J Cancer12: 438 (1973) Brain Male BW1 CVCL_0021
Cancer cell line
U-138 MG Glioblastoma cell line Ponten et al., Acta Pathol Microbiol Scand 74: 465 (1968) Brain Male, 47 DSMZ CVCL_0020
Cancer cell line
SH-SY5Y Metastatic neuroblastoma, clonal subline of neuroepithelioma cell line SK-N-SH Biedler et al., Cancer Res 33: 2643 (1973) Bone marrow Female, 4 DSMZ CVCL_0019
Cancer cell line

Myeloid

HL-60 Acute promyelocytic leukemia (APL) cell line Collins et al., Nature 270: 347-349 (1977) Peripheral blood Female, 35 DSMZ CVCL_0002
Cancer cell line
HEL Erythroleukemia cell line (AML M6 in relapse after treatment for Hodgkin's disease) Martin et al., Science 216: 1233 (1982) Peripheral blood Male, 30 DSMZ CVCL_0001
Cancer cell line
HMC-1 Mastcell leukemia cell line Butterfield et al., Leukemia Res. 12: 345-355 (1988) Peripheral blood Male, 52 KN2 CVCL_0003
Cancer cell line
K-562 Chronic myeloid leukemia (CML) cell line Lozzio et al., J Natl Cancer Inst 50: 535 (1973) Pleural effusion Female, 53 DSMZ CVCL_0004
Cancer cell line
NB-4 Acute promyelocytic leukemia (APL) cell line Lanotte et al., Blood 77: 1080 (1991) Bone marrow Female, 23 DSMZ CVCL_0005
Cancer cell line
THP-1 Acute monocytic leukemia (AML) cell line Tsuchiya et al., Int J Cancer 26: 171 (1980) Peripheral blood Male, 1 DSMZ CVCL_0006
Cancer cell line
U-937 Myelomonocytic histiocytic lymphoma cell line Sundström C, Nilsson K., Int J Cancer 17:565-577 (1976) Pleural effusion Male, 37 KN2 CVCL_0007
Cancer cell line

Lymphoid

HDLM-2 Hodgkin lymphoma cell line Drexler et al., Leuk Res 10: 487 (1986) Pleural effusion Male, 74 DSMZ CVCL_0009
Cancer cell line
U-698 B-cell lymphoma cell line (lymphoblastic lymphosarcoma) Nilsson et al., Int J Cancer 13: 808-823 (1974) Tonsil Male, 7 DSMZ CVCL_0017
Cancer cell line
U-266/84 Multiple myeloma cell line (1984, in vitro differentiated) Hellman et al., Eur J Immunol 18:905-910 (1988) Peripheral blood Male, 53 KN2 CVCL_0016
Cancer cell line
U-266/70 Multiple myeloma cell line (1970, IL-6-dependent) Nilsson et al Clin Exp Immunol 7:477-489 (1970) Peripheral blood Male, 53 KN2 CVCL_0015
Cancer cell line
RPMI-8226 Multiple myeloma cell line Matsuoka et al., Proc Soc Exp Biol Med 125: 1246 (1967) Peripheral blood Male, 61 DSMZ CVCL_0014
Cancer cell line
REH** Pre-B cell leukemia cell line (ALL, first relapse) Rosenfeld et al., Nature 267: 841–3 (1977) Peripheral blood Female, 15 DSMZ CVCL_1650
Cancer cell line
MOLT-4 Acute lymphoblastic leukemia (T-ALL) cell line Minowada et al., J Natl Cancer Inst 49: 891 (1972) Peripheral blood Male, 19 DSMZ CVCL_0013
Cancer cell line
LP-1 Multiple myeloma cell line Pegoraro et al., Blood 73: 1020 (1989) Peripheral blood Female, 56 DSMZ CVCL_0012
Cancer cell line
Karpas-707 Multiple myeloma cell line Karpas et al., Lancet Apr 24;1(8278): 931-3 (1982) Bone marrow and peripheral blood Male, 53 KN2 CVCL_0010
Cancer cell line
Daudi Human Burkitt lymphoma cell line Klein et al., Cancer Res 28: 1300 (1968) Orbital biopsy Male, 16 DSMZ CVCL_0008
Cancer cell line

Lung

A549 Lung carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Lung Male, 58 DSMZ CVCL_0023
Cancer cell line
SCLC-21H Small cell lung carcinoma cell line Bepler et al., J Cancer Res Clin Oncol 113: 31 (1987) Pleural effusion Male, 46 DSMZ CVCL_0024
Cancer cell line

Abdominal

CACO-2 Colon adenocarcinoma cell line Fogh J., et al. J. Natl. Cancer Inst. 58: 209 (1977) Colon Male, 72 DSMZ CVCL_0025
Cancer cell line
CAPAN-2 Pancreas adenocarcinoma cell line Kyriazis et al., Cancer Res 46: 5810 (1986) Pancreas Male, 56 DSMZ CVCL_0026
Cancer cell line
Hep G2 Hepatocellular carcinoma cell line Aden et al., Nature 282: 615 (1979) Liver Male, 15 DSMZ CVCL_0027
Cancer cell line

Breast, female reproductive system

SK-BR-3 Metastatic breast adenocarcinoma cell line Fogh J, editor. Human tumor cells in vitro. New York: Plenum Press (1975) Pleural effusion Female, 43 LGC/ATCC CVCL_0033
Cancer cell line
SiHa Cervical squamous cell carcinoma cell line, integrated 1-2 copies of HPV16 Friedl F. et al.Proc., Soc. Exp. Biol. Med. 135: 543-545 (1970) Cervix Female, 55 LGC/ATCC CVCL_0032
Cancer cell line
MCF7 Metastatic breast adenocarcinoma cell line Soule et al., J Natl Cancer Inst 51: 1409 (1973) Pleural effusion Female, 69 DSMZ CVCL_0031
Cancer cell line
EFO-21 Metastatic ovarian serous cystadenocarcinoma cell line Simon et al., J Natl Cancer Inst 70: 839 (1983) Ascites Female, 56 DSMZ CVCL_0029
Cancer cell line
AN3-CA Metastatic endometrial adenocarcinoma cell line Dawe et al., J Natl Cancer Inst 33: 441 (1964) Lymph node Female, 55 DSMZ CVCL_0028
Cancer cell line
HeLa Cervical epithelial adenocarcinoma cell line Scherer et al., J Exp Med 97: 695 (1953) Cervix Female, 31 DSMZ CVCL_0030
Cancer cell line

Renal, urinary, male reproductive system

HEK 293 Embryonal kidney cell line, transformed by adenovirus type 5 Graham et al., J Gen Virol 36: 59 (1977) Embryonal kidney Female DSMZ CVCL_0045
Transformed cell line
NTERA-2 Metastatic embryonal carcinoma cell line, cloned from TERA-2 Fogh & Trempe, Human Tumor Cells in Vitro : 115 (1975) Male, 22 DSMZ CVCL_0034
Cancer cell line
PC-3 Metastatic poorly differentiated prostate adenocarcinoma cell line Kaighn et al., Invest Urol 17: 16-23 (1979) Bone marrow Male, 62 DSMZ CVCL_0035
Cancer cell line
RT4 Urinary bladder transitional cell carcinoma cell line Rigby et al., Br J Cancer 24: 746 (1970) Urinary bladder Male, 63 DSMZ/ECACC CVCL_0036
Cancer cell line
RPTEC TERT1 Proximal tubular epithelial cell line Wieser et al., Am J Physiol Renal Physiol 295(5): 1365-1375 (2008) Human kidney cortex Male Evercyte CVCL_K278
Telomerase immortalized cell line

Skin

A-431 Epidermoid carcinoma cell line Giard et al., J Natl Cancer Inst 51: 1417 (1973) Skin Female, 85 DSMZ CVCL_0037
Cancer cell line
HaCaT Keratinocyte cell line Boukamp et al., J Cell Biol Mar 106(3): 761 (1988) Skin Male NEF3 CVCL_0038
Spontaneously immortalized cell line
SK-MEL-30 Metastatic malignant melanoma cell line Carey et al., Proc Natl Acad Sci USA 73: 3278 (1976) Subcutis Male, 67 DSMZ CVCL_0039
Cancer cell line
WM-115 Malignant melanoma cell line Westermark B. et al, Proc Natl Acad Sci U S A. Oct; 83(19): 7197 (1986) Skin Female, 58 BW1 CVCL_0040
Cancer cell line

Sarcoma

U-2197 Malignant fibrous histiocytoma cell line Genberg et al., Am J Pathol 135: 1185 (1989) Subcutis Male, 79 DSMZ CVCL_0043
Cancer cell line
LHCN-M2 Adherent myoblast cell line Zhu et al., Aging Cell 6(4): 515-523 (2007) Satellite cells (from pectoralis major muscle) Male, 41 Evercyte CVCL_8890
Telomerase immortalized cell line
ASC TERT1 Adipose tissue-derived mesenchymal stem cell line Wolbank S. et al., Tissue Eng Part A: Jul;15(7):1843-54 (2009) Adipose tissue Female Evercyte CVCL_U602
Telomerase immortalized cell line
U-2 OS Osteosarcoma cell line Ponten J., Saksela E. Int. J. Cancer 2: 434 (1967) Bone Female, 15 LGC/ATCC CVCL_0042
Cancer cell line
RH-30 Metastatic rhabdomyosarcoma cell line Douglass et al., Cytogenet Cell Genet 45: 148-155 (1987) Bone marrow Male, 17 DSMZ CVCL_0041
Cancer cell line

Fibroblast

HBF TERT88 Adherent brain fibroblast cell line NA Brain Female Evercyte CVCL_9Q52
Telomerase immortalized cell line
BJ Adherent fibroblast cell line Bodnar AG., et al. Science 279: 349-352 (1998) Foreskin Male LGC/ATCC CVCL_3653
Finite cell line
BJ hTERT+ Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT Bodnar et al., Science 279(5349): 349-352 (1998) Foreskin Male NA CVCL_6573
Telomerase immortalized cell line
BJ hTERT+ SV40 Large T+ Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT, transformed with SV40 Large-T infection Bodnar et al., Science 279(5349): 349-352 (1998) Foreskin Male NA
BJ hTERT+ SV40 Large T+ RasG12V Adherent BJ fibroblast cell line immortalized with ectopic expression of hTERT, transformed with SV40 Large-T infection, made metastasizing with oncogenic H-Ras Bodnar et al., Science 279(5349): 349-352 (1998) Foreskin Male NA

Endothelial

TIME Telomerase-immortalized human microvascular endothelial cells (pooled) Venetsanakos et el., Exp Cell Res., Feb 1;273(1): 21-33 (2002) Skin Male LCW54 CVCL_0047
Telomerase immortalized cell line
HUVEC TERT2 Umbilical vein endothelial cell line NA Umbilical vein Female Evercyte CVCL_9Q53
Telomerase immortalized cell line

Miscellaneous

HTh 83 Anaplastic thyroid carcinoma cell line Dahlman et al., J. Pathol. 191: 376-386 (2000) Thyroid Male, 67 NEH5 CVCL_0046
Cancer cell line
HTCEpi Telomerase-immortalized corneal epithelial cell line Robertson et al., Invest Ophthalmol Vis Sci 46(2): 470-478 (2005) Cornea Male, 62 Evercyte CVCL_AQ44
Telomerase immortalized cell line
BEWO Metastatic choriocarcinoma cell line Pattillo et al., Cancer Res 28: 1231-1236 (1968) Brain Male DSMZ CVCL_0044
Cancer cell line

Mouse

NIH 3T3 Mouse embryonal fibroblast cell line Aaronson et al., J Cell Physio 72(2): 141-148 (1968) Embryo DSMZ CVCL_0594
Spontaneously immortalized cell line

Notes

*
Allen M, Bjerke M, Edlund H, Nelander S and Westermark B: Origin of the U87MG glioma cell line: Good news and bad news. Science Translational Medicine 31 Aug 2016: Vol. 8, Issue 354, pp. 354. DOI: 10.1126/scitranslmed.aaf6853
**
REH was previously listed as KM3. Due to misidentification, KM3 has been deleted from the DSMZ catalogue and the true identity is REH. DSMZ - False leukemia and lymphoma cell lines. Drexler et al., False leukemia-lymphoma cell lines: An update on over 500 cell lines. Leukemia 17: 416-426 (2003)

Source information

  1. Prof. Bengt Westermark, Uppsala University, Uppsala, Sweden
  2. Prof. Kenneth Nilsson, Uppsala University, Uppsala, Sweden
  3. Prof. Norbert E Fusenig, DKFZ, Heidelberg, Germany
  4. Prof. Martin McMahon, UCSF Comprehensive Cancer Center, San Fransisco, USA
  5. Assoc. Prof. Nis-Erik Heldin, Uppsala University, Uppsala, Sweden

Hematopoietic cell lines


Schematic description of hematopoietic differentiation lineages, and cellular origin for hematopoietic cell lines.