Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of cytoplasm in human cell line U-2 OS (HPA003570)
Scale bar represents 10µm
The cytosol is referred to as the part of the cytoplasm that is not a membrane bound organelle or a cytoskeleton. It is a liquid matrix embedding the organelles, making up about 70% of the cell volume and is mainly composed of water. The cytosol plays an important role in the cell homeostasis as the site of many metabolic reactions and in the storage of free proteins as well as ions such as potassium, sodium and bicarbonate.
The immunofluorescent staining of the cytoplasm encompasses the whole cell but the nucleus. The characteristic of the staining can vary from smooth to granular and is often stronger closer to the nucleus.