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Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of nuclear speckles in human cell line A-431 (HPA041411)
Scale bar represents 10µm
Nuclear speckles
Nuclear speckles are a non-membrane-bound nuclear sub-compartment that contains pre-mRNA splicing factors. Besides the storage of those splicing factors nuclear speckles are thought to serve as a regulatory site for transcription.
Immunofluorescent staining
Nuclear speckles appear as an irregular, mottled staining pattern inside the nucleoplasm.