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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Antibody dilution
1:125
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA045383
Antibody HPA072477
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA072477 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA045383 across 72 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells and strong cytoplasmic positivity in islets of Langerhans.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells and strong cytoplasmic positivity in islets of Langerhans.
Expression
RNA: detected in 37 tissues Protein: detected in 68 cell types
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:600
1:700
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045383
Antibody HPA072477
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.9
61.9
Antibody dilution
1:250
1:610
PROTEIN ARRAY
Antibody HPA045383
Antibody HPA072477
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.