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Antibody staining overlaps with antibody HPA037504.
Validated
Antibody staining overlaps with antibody HPA037503.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nuclear bodies.
Antibody dilution
1:7
1:54
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
Validation: Genetic
Supported
Significant downregulation 10-25% by one/two siRNA:s
Figure description
Nuclear region of segmented cells in 10x-images
Antibody dilution
1:54
IMMUNOHISTOCHEMISTRY
Antibody HPA037503
Antibody HPA037504
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong nuclear positivity in seminiferus tubules.
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 36 cell types
RNA: detected in 37 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:13
1:125
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA037503
Antibody HPA037504
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414063)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414063)
Target mass (kDa)
79.7
79.7, 61.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037503
Antibody HPA037504
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.