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Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli fibrillar center.
Antibody dilution
1:15
1:7
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA015313
Antibody HPA017587
Standard validation
Supported
Figure description
Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in cells in molecular layer.
Expression
RNA: detected in 34 tissues Protein: detected in 5 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015313
Antibody HPA017587
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402788)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil