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Immunohistochemical staining of human heart muscle shows distinct positivity in intercalated discs.
Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 26 cell types
RNA: detected in 37 tissues Protein: detected in 12 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:400
1:125
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019826
Antibody HPA049622
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415615)
Target mass (kDa)
27.4, 16.9
27.4, 16.9
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA019826
Antibody HPA049622
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.