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Immunohistochemical staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
Expression
RNA: detected in 14 tissues Protein: detected in 14 cell types
RNA: detected in 14 tissues Protein: detected in 15 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001893
Antibody CAB058690
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401814)
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
32.2
32.2, 12
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA001893
Antibody CAB058690
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.