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Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.
Antibody dilution
1:65
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA051207
Antibody HPA064657
Standard validation
Approved
Figure description
Immunohistochemical staining of human small intestine shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 21 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
Antibody dilution
1:40
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051207
Antibody HPA064657
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408690)
Target mass (kDa)
55.5, 36.8
55.5, 36.8
Antibody dilution
1:125
1:220
PROTEIN ARRAY
Antibody HPA051207
Antibody HPA064657
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.