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Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cell junctions.
Antibody dilution
1:97
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA034992
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic positivity(granular pattern) in glandular cells.
Expression
RNA: detected in 32 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
Antibody dilution
1:850
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA034992
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407143)
Target mass (kDa)
47.4, 42.8, 39.4, 33.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA034992
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.