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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.
Antibody dilution
1:84
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA044607
Antibody CAB004985
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows distinct cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 47 cell types
RNA: detected in 37 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:170
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA044607
Antibody CAB004985
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412010)
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
28.9, 19.2, 16.5, 12.6
28.9, 23.4, 19.2, 16.5, 12.6
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA044607
Antibody CAB004985
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.