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RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue/cell line enriched, group enriched, tissue/cell line enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for The Cancer Genome Atlas (TCGA) data from cancer tissues and internally generated Human Protein Atlas (HPA) data from normal tissues and cell lines.
TCGA (cancer tissue):
Expressed in all
HPA (cell line):
Cell line enriched (A-431)
HPA (normal tissue):
Tissue enhanced (placenta)
Protein evidencei
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Protein expression normal tissuei
A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.
"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.
Cytoplasmic and membranous expression in several tissues, most abundant in placenta.
ANTIBODY IHC RELIABILITY
Data reliability descriptioni
Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.
Antibody staining mainly consistent with RNA expression data.
Reliability scorei
Reliability score (score description), divided into Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between the staining pattern of one antibody or several antibodies with RNA-seq data and available gene/protein characterization data.
Kaplan-Meier plots for all cancers where high expression of this gene has significant (p<0.001) association with patient survival are shown in this summary. Whether the prognosis is favourable or unfavourable is indicated in brackets. Each Kaplan-Meier plot is clickable and redirects to a detailed page that includes individual expression and survival data for patients with the selected cancer.
RNA expression overview shows RNA-seq data from The Cancer Genome Atlas (TCGA).
TCGA dataseti
RNA-seq data in 17 cancer types are reported as median FPKM (number Fragments Per Kilobase of exon per Million reads), generated by the The Cancer Genome Atlas (TCGA). RNA cancer tissue category is calculated based on mRNA expression levels across all 17 cancer tissues and include: cancer tissue enriched, cancer group enriched, cancer tissue enhanced, expressed in all, mixed and not detected. To access cancer specific RNA and prognostic data, click on the cancer name.
Antibody staining in 20 different cancers is summarized by a selection of four standard cancer tissue samples representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional fifth image can be added as a complement. The assay and annotation is described here. Note that samples used for immunohistochemistry by the Human Protein Atlas do not correspond to samples in the TCGA dataset.
For each cancer, the fraction of samples with protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the left). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The mouse-over function displays additional data for the features in the staining summary view.
Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.
A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.
Most malignancies showed weak to moderate cytoplasmic immunoreactivity. Malignant gliomas displayed moderate to strong positivity. A majority of cases in malignant lymphomas, breast, prostate and ovarian cancers were negative.
A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.
A majority of urothelial, cervical, skin, head and neck, and lung cancers showed moderate membranous and cytoplasmic positivity. Most remaining cancer cells were weakly stained or negative.
GENE INFORMATIONi
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
Gene name
EGFR (HGNC Symbol)
Synonyms
ERBB, ERBB1
Description
Epidermal growth factor receptor (HGNC Symbol)
Entrez gene summary
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
EGFR-001
EGFR-002
EGFR-003
EGFR-004
EGFR-005
EGFR-009
EGFR-201
EGFR-202
PROTEIN INFORMATIONi
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Enzymes ENZYME proteins Transferases Kinases Tyr protein kinases Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins RAS pathway related proteins Cancer-related genes Candidate cancer biomarkers Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Amplifications COSMIC Somatic Mutations COSMIC Other Mutations COSMIC Missense Mutations COSMIC Germline Mutations Disease related genes FDA approved drug targets Biotech drugs Small molecule drugs Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane] GO:0000165 [MAPK cascade] GO:0000186 [activation of MAPKK activity] GO:0001503 [ossification] GO:0001934 [positive regulation of protein phosphorylation] GO:0003682 [chromatin binding] GO:0003690 [double-stranded DNA binding] GO:0004709 [MAP kinase kinase kinase activity] GO:0004713 [protein tyrosine kinase activity] GO:0004714 [transmembrane receptor protein tyrosine kinase activity] GO:0004716 [receptor signaling protein tyrosine kinase activity] GO:0004888 [transmembrane signaling receptor activity] GO:0005006 [epidermal growth factor-activated receptor activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005615 [extracellular space] GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0005768 [endosome] GO:0005789 [endoplasmic reticulum membrane] GO:0005886 [plasma membrane] GO:0005925 [focal adhesion] GO:0006468 [protein phosphorylation] GO:0006950 [response to stress] GO:0007165 [signal transduction] GO:0007166 [cell surface receptor signaling pathway] GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway] GO:0007173 [epidermal growth factor receptor signaling pathway] GO:0007202 [activation of phospholipase C activity] GO:0007264 [small GTPase mediated signal transduction] GO:0007265 [Ras protein signal transduction] GO:0007411 [axon guidance] GO:0007611 [learning or memory] GO:0008283 [cell proliferation] GO:0008284 [positive regulation of cell proliferation] GO:0008286 [insulin receptor signaling pathway] GO:0008543 [fibroblast growth factor receptor signaling pathway] GO:0009986 [cell surface] GO:0010008 [endosome membrane] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016323 [basolateral plasma membrane] GO:0016337 [single organismal cell-cell adhesion] GO:0018108 [peptidyl-tyrosine phosphorylation] GO:0019899 [enzyme binding] GO:0019903 [protein phosphatase binding] GO:0030122 [AP-2 adaptor complex] GO:0030235 [nitric-oxide synthase regulator activity] GO:0030335 [positive regulation of cell migration] GO:0031625 [ubiquitin protein ligase binding] GO:0031659 [positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle] GO:0031901 [early endosome membrane] GO:0031965 [nuclear membrane] GO:0035413 [positive regulation of catenin import into nucleus] GO:0038095 [Fc-epsilon receptor signaling pathway] GO:0042059 [negative regulation of epidermal growth factor receptor signaling pathway] GO:0042177 [negative regulation of protein catabolic process] GO:0042327 [positive regulation of phosphorylation] GO:0042802 [identical protein binding] GO:0043006 [activation of phospholipase A2 activity by calcium-mediated signaling] GO:0043066 [negative regulation of apoptotic process] GO:0043235 [receptor complex] GO:0043406 [positive regulation of MAP kinase activity] GO:0045087 [innate immune response] GO:0045121 [membrane raft] GO:0045429 [positive regulation of nitric oxide biosynthetic process] GO:0045739 [positive regulation of DNA repair] GO:0045740 [positive regulation of DNA replication] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0046777 [protein autophosphorylation] GO:0046982 [protein heterodimerization activity] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0048011 [neurotrophin TRK receptor signaling pathway] GO:0048015 [phosphatidylinositol-mediated signaling] GO:0050679 [positive regulation of epithelial cell proliferation] GO:0050730 [regulation of peptidyl-tyrosine phosphorylation] GO:0050999 [regulation of nitric-oxide synthase activity] GO:0051015 [actin filament binding] GO:0051205 [protein insertion into membrane] GO:0051897 [positive regulation of protein kinase B signaling] GO:0070141 [response to UV-A] GO:0070374 [positive regulation of ERK1 and ERK2 cascade] GO:0070435 [Shc-EGFR complex] GO:0071364 [cellular response to epidermal growth factor stimulus] GO:0071392 [cellular response to estradiol stimulus] GO:0097489 [multivesicular body, internal vesicle lumen]
Enzymes ENZYME proteins Transferases Kinases Tyr protein kinases Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins RAS pathway related proteins Cancer-related genes Candidate cancer biomarkers Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Amplifications COSMIC Somatic Mutations COSMIC Other Mutations COSMIC Missense Mutations COSMIC Germline Mutations Disease related genes FDA approved drug targets Biotech drugs Small molecule drugs Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane] GO:0000165 [MAPK cascade] GO:0000186 [activation of MAPKK activity] GO:0001503 [ossification] GO:0001934 [positive regulation of protein phosphorylation] GO:0003682 [chromatin binding] GO:0003690 [double-stranded DNA binding] GO:0004709 [MAP kinase kinase kinase activity] GO:0004713 [protein tyrosine kinase activity] GO:0004714 [transmembrane receptor protein tyrosine kinase activity] GO:0004716 [receptor signaling protein tyrosine kinase activity] GO:0004888 [transmembrane signaling receptor activity] GO:0005006 [epidermal growth factor-activated receptor activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005615 [extracellular space] GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0005768 [endosome] GO:0005789 [endoplasmic reticulum membrane] GO:0005886 [plasma membrane] GO:0005925 [focal adhesion] GO:0006468 [protein phosphorylation] GO:0006950 [response to stress] GO:0007165 [signal transduction] GO:0007166 [cell surface receptor signaling pathway] GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway] GO:0007173 [epidermal growth factor receptor signaling pathway] GO:0007202 [activation of phospholipase C activity] GO:0007264 [small GTPase mediated signal transduction] GO:0007265 [Ras protein signal transduction] GO:0007411 [axon guidance] GO:0007611 [learning or memory] GO:0008283 [cell proliferation] GO:0008284 [positive regulation of cell proliferation] GO:0008286 [insulin receptor signaling pathway] GO:0008543 [fibroblast growth factor receptor signaling pathway] GO:0009986 [cell surface] GO:0010008 [endosome membrane] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016323 [basolateral plasma membrane] GO:0016337 [single organismal cell-cell adhesion] GO:0018108 [peptidyl-tyrosine phosphorylation] GO:0019899 [enzyme binding] GO:0019903 [protein phosphatase binding] GO:0030122 [AP-2 adaptor complex] GO:0030235 [nitric-oxide synthase regulator activity] GO:0030335 [positive regulation of cell migration] GO:0031625 [ubiquitin protein ligase binding] GO:0031659 [positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle] GO:0031901 [early endosome membrane] GO:0031965 [nuclear membrane] GO:0035413 [positive regulation of catenin import into nucleus] GO:0038095 [Fc-epsilon receptor signaling pathway] GO:0042059 [negative regulation of epidermal growth factor receptor signaling pathway] GO:0042177 [negative regulation of protein catabolic process] GO:0042327 [positive regulation of phosphorylation] GO:0042802 [identical protein binding] GO:0043006 [activation of phospholipase A2 activity by calcium-mediated signaling] GO:0043066 [negative regulation of apoptotic process] GO:0043235 [receptor complex] GO:0043406 [positive regulation of MAP kinase activity] GO:0045087 [innate immune response] GO:0045121 [membrane raft] GO:0045429 [positive regulation of nitric oxide biosynthetic process] GO:0045739 [positive regulation of DNA repair] GO:0045740 [positive regulation of DNA replication] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0046777 [protein autophosphorylation] GO:0046982 [protein heterodimerization activity] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0048011 [neurotrophin TRK receptor signaling pathway] GO:0048015 [phosphatidylinositol-mediated signaling] GO:0050679 [positive regulation of epithelial cell proliferation] GO:0050730 [regulation of peptidyl-tyrosine phosphorylation] GO:0050999 [regulation of nitric-oxide synthase activity] GO:0051015 [actin filament binding] GO:0051205 [protein insertion into membrane] GO:0051897 [positive regulation of protein kinase B signaling] GO:0070141 [response to UV-A] GO:0070374 [positive regulation of ERK1 and ERK2 cascade] GO:0070435 [Shc-EGFR complex] GO:0071364 [cellular response to epidermal growth factor stimulus] GO:0071392 [cellular response to estradiol stimulus] GO:0097489 [multivesicular body, internal vesicle lumen]
Enzymes ENZYME proteins Transferases Kinases Tyr protein kinases Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins RAS pathway related proteins Cancer-related genes Candidate cancer biomarkers Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Amplifications COSMIC Somatic Mutations COSMIC Other Mutations COSMIC Missense Mutations COSMIC Germline Mutations Disease related genes FDA approved drug targets Biotech drugs Small molecule drugs Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane] GO:0000165 [MAPK cascade] GO:0000186 [activation of MAPKK activity] GO:0001503 [ossification] GO:0001934 [positive regulation of protein phosphorylation] GO:0003682 [chromatin binding] GO:0003690 [double-stranded DNA binding] GO:0004709 [MAP kinase kinase kinase activity] GO:0004713 [protein tyrosine kinase activity] GO:0004714 [transmembrane receptor protein tyrosine kinase activity] GO:0004716 [receptor signaling protein tyrosine kinase activity] GO:0004888 [transmembrane signaling receptor activity] GO:0005006 [epidermal growth factor-activated receptor activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005615 [extracellular space] GO:0005634 [nucleus] GO:0005737 [cytoplasm] GO:0005768 [endosome] GO:0005789 [endoplasmic reticulum membrane] GO:0005886 [plasma membrane] GO:0005925 [focal adhesion] GO:0006468 [protein phosphorylation] GO:0006950 [response to stress] GO:0007165 [signal transduction] GO:0007166 [cell surface receptor signaling pathway] GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway] GO:0007173 [epidermal growth factor receptor signaling pathway] GO:0007202 [activation of phospholipase C activity] GO:0007264 [small GTPase mediated signal transduction] GO:0007265 [Ras protein signal transduction] GO:0007411 [axon guidance] GO:0007611 [learning or memory] GO:0008283 [cell proliferation] GO:0008284 [positive regulation of cell proliferation] GO:0008286 [insulin receptor signaling pathway] GO:0008543 [fibroblast growth factor receptor signaling pathway] GO:0009986 [cell surface] GO:0010008 [endosome membrane] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016323 [basolateral plasma membrane] GO:0016337 [single organismal cell-cell adhesion] GO:0018108 [peptidyl-tyrosine phosphorylation] GO:0019899 [enzyme binding] GO:0019903 [protein phosphatase binding] GO:0030122 [AP-2 adaptor complex] GO:0030235 [nitric-oxide synthase regulator activity] GO:0030335 [positive regulation of cell migration] GO:0031625 [ubiquitin protein ligase binding] GO:0031659 [positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G1/S transition of mitotic cell cycle] GO:0031901 [early endosome membrane] GO:0031965 [nuclear membrane] GO:0035413 [positive regulation of catenin import into nucleus] GO:0038095 [Fc-epsilon receptor signaling pathway] GO:0042059 [negative regulation of epidermal growth factor receptor signaling pathway] GO:0042177 [negative regulation of protein catabolic process] GO:0042327 [positive regulation of phosphorylation] GO:0042802 [identical protein binding] GO:0043006 [activation of phospholipase A2 activity by calcium-mediated signaling] GO:0043066 [negative regulation of apoptotic process] GO:0043235 [receptor complex] GO:0043406 [positive regulation of MAP kinase activity] GO:0045087 [innate immune response] GO:0045121 [membrane raft] GO:0045429 [positive regulation of nitric oxide biosynthetic process] GO:0045739 [positive regulation of DNA repair] GO:0045740 [positive regulation of DNA replication] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0046777 [protein autophosphorylation] GO:0046982 [protein heterodimerization activity] GO:0048010 [vascular endothelial growth factor receptor signaling pathway] GO:0048011 [neurotrophin TRK receptor signaling pathway] GO:0048015 [phosphatidylinositol-mediated signaling] GO:0050679 [positive regulation of epithelial cell proliferation] GO:0050730 [regulation of peptidyl-tyrosine phosphorylation] GO:0050999 [regulation of nitric-oxide synthase activity] GO:0051015 [actin filament binding] GO:0051205 [protein insertion into membrane] GO:0051897 [positive regulation of protein kinase B signaling] GO:0070141 [response to UV-A] GO:0070374 [positive regulation of ERK1 and ERK2 cascade] GO:0070435 [Shc-EGFR complex] GO:0071364 [cellular response to epidermal growth factor stimulus] GO:0071392 [cellular response to estradiol stimulus] GO:0097489 [multivesicular body, internal vesicle lumen]
Predicted intracellular proteins RAS pathway related proteins Cancer-related genes Mutated cancer genes Mutational cancer driver genes COSMIC somatic mutations in cancer genes COSMIC Amplifications COSMIC Somatic Mutations COSMIC Other Mutations COSMIC Missense Mutations COSMIC Germline Mutations Protein evidence (Ezkurdia et al 2014)