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Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Antibody dilution
1:8
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA040758
Antibody HPA064645
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in astrocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 56 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA040758
Antibody HPA064645
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407018)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
86.3
86.3
Antibody dilution
1:125
1:1580
PROTEIN ARRAY
Antibody HPA040758
Antibody HPA064645
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.