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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.
Immunofluorescent staining of human cell line SiHa shows localization to endoplasmic reticulum.
Antibody dilution
1:132
1:122
Literature conformity
The subcellular location is not consistent with literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA051849
Antibody HPA058348
Antibody HPA070542
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human kidney shows strong membranous positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 26 cell types
RNA: detected in 37 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:350
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA051849
Antibody HPA058348
Antibody HPA070542
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403416)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
51.3
15.3
51.3
Antibody dilution
1:250
1:80
1:400
PROTEIN ARRAY
Antibody HPA051849
Antibody HPA058348
Antibody HPA070542
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.