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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Antibody dilution
1:200
1:30
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA034569
Antibody HPA054975
Antibody CAB011464
Antibody CAB035990
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of non-germinal center cells.
Expression
RNA: detected in 29 tissues Protein: detected in 68 cell types
RNA: detected in 29 tissues Protein: detected in 75 cell types
RNA: detected in 29 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:800
1:75
1:130
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034569
Antibody HPA054975
Antibody CAB011464
Antibody CAB035990
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
19.7, 15.8
19.7, 15.8
19.7, 17.4, 16.7, 15.8, 14.4, 8.1, 5.1
19.7, 17.4, 16.7, 15.8, 14.4, 8.1, 5.1
Antibody dilution
1:250
1:560
1:500
1:500
PROTEIN ARRAY
Antibody HPA034569
Antibody HPA054975
Antibody CAB011464
Antibody CAB035990
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.