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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
Antibody dilution
1:38
1:72
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA014324
Antibody HPA051564
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in glial cells.
Immunohistochemical staining of human hippocampus shows moderate cytoplasmic positivity in astrocytes.
Expression
RNA: detected in 36 tissues Protein: detected in 10 cell types
RNA: detected in 36 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:60
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA014324
Antibody HPA051564
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407602)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407602)
Target mass (kDa)
30.8, 30.3
30.8, 30.3, 20
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA014324
Antibody HPA051564
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.