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Immunofluorescent staining of human cell line BJ shows localization to endoplasmic reticulum.
Antibody dilution
1:149
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA017913
Antibody HPA017915
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human epididymis shows distinct positivity of collagen fibers and endothelial cells in the stroma.
Immunohistochemical staining of human testis shows strong positivity of collagen fibers and endothelial cells in the stroma.
Expression
RNA: detected in 35 tissues Protein: detected in 46 cell types
RNA: detected in 35 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017913
Antibody HPA017915
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
141.7, 140.1
141.7, 140.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA017913
Antibody HPA017915
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.