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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PLD1
  • TISSUE

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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

PLD1

Gene description

Phospholipase D1, phosphatidylcholine-specific

Protein class

Enzymes
Plasma proteins
Predicted intracellular proteins
RAS pathway related proteins

Predicted localization

Intracellular

Number of transcripts

6
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (HUVEC TERT2, TIME)
HPA (normal tissue):Tissue enhanced (gallbladder)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression at variable levels in most tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Pending external verification.

Reliability score

Approved based on 2 antibodies.
HPA042396 , CAB004527
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PROGNOSTIC SUMMARY

Prognostic marker in urothelial cancer (unfavourable).
Urothelial cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA042396
CAB004527

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancers generally displayed weak to moderate cytoplasmic and/or membranous staining. Strong staining was observed in cases of melanomas, colorectal, cervical, skin and pancreatic cancers. Testicular cancers along with several endometrial cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong nucleolar immunoreactivity was observed in most cancers. Additional cytoplasmic or membranous positivity was observed in a few cases.

GENE INFORMATION

Gene name

PLD1

Synonyms

Description

Phospholipase D1, phosphatidylcholine-specific (HGNC Symbol)

Entrez gene summary

This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]

Chromosome

3

Cytoband

q26.31

Chromosome location (bp)

171600405 - 171810950

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000075651 (version 83.38)

Entrez gene

5337

UniProt

Q13393 (UniProt - Evidence at protein level)

neXtProt

NX_Q13393

Antibodypedia

PLD1 antibodies

PROTEIN BROWSER

PLD1-001
PLD1-002
PLD1-003
PLD1-010
PLD1-201
PLD1-202

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

PLD1-001
ENSP00000342793
ENST00000351298
Q13393 [Direct mapping]
Phospholipase D1
Show all
Enzymes
   ENZYME proteins
   Hydrolases
Predicted intracellular proteins
Plasma proteins
RAS pathway related proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0003824 [catalytic activity]
GO:0004630 [phospholipase D activity]
GO:0005515 [protein binding]
GO:0005765 [lysosomal membrane]
GO:0005768 [endosome]
GO:0005789 [endoplasmic reticulum membrane]
GO:0005794 [Golgi apparatus]
GO:0006643 [membrane lipid metabolic process]
GO:0006644 [phospholipid metabolic process]
GO:0006654 [phosphatidic acid biosynthetic process]
GO:0006655 [phosphatidylglycerol biosynthetic process]
GO:0006887 [exocytosis]
GO:0006935 [chemotaxis]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0009395 [phospholipid catabolic process]
GO:0016020 [membrane]
GO:0016324 [apical plasma membrane]
GO:0030139 [endocytic vesicle]
GO:0030334 [regulation of cell migration]
GO:0031902 [late endosome membrane]
GO:0032534 [regulation of microvillus assembly]
GO:0035091 [phosphatidylinositol binding]
GO:0044281 [small molecule metabolic process]
GO:0046473 [phosphatidic acid metabolic process]
GO:0046474 [glycerophospholipid biosynthetic process]
GO:0048017 [inositol lipid-mediated signaling]
GO:0048471 [perinuclear region of cytoplasm]
GO:0048870 [cell motility]
GO:0050764 [regulation of phagocytosis]
GO:0050830 [defense response to Gram-positive bacterium]
GO:0060627 [regulation of vesicle-mediated transport]
GO:0070290 [N-acylphosphatidylethanolamine-specific phospholipase D activity]
Show all
1074 aa
124.2 kDa
No 0
PLD1-002
ENSP00000348681
ENST00000356327
Q13393 [Direct mapping]
Phospholipase D1
Show all
Enzymes
   ENZYME proteins
   Hydrolases
Predicted intracellular proteins
Plasma proteins
RAS pathway related proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0003824 [catalytic activity]
GO:0004630 [phospholipase D activity]
GO:0005515 [protein binding]
GO:0005765 [lysosomal membrane]
GO:0005768 [endosome]
GO:0005789 [endoplasmic reticulum membrane]
GO:0005794 [Golgi apparatus]
GO:0006643 [membrane lipid metabolic process]
GO:0006644 [phospholipid metabolic process]
GO:0006654 [phosphatidic acid biosynthetic process]
GO:0006655 [phosphatidylglycerol biosynthetic process]
GO:0006887 [exocytosis]
GO:0006935 [chemotaxis]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0009395 [phospholipid catabolic process]
GO:0016020 [membrane]
GO:0016324 [apical plasma membrane]
GO:0030139 [endocytic vesicle]
GO:0030334 [regulation of cell migration]
GO:0031902 [late endosome membrane]
GO:0032534 [regulation of microvillus assembly]
GO:0035091 [phosphatidylinositol binding]
GO:0044281 [small molecule metabolic process]
GO:0046473 [phosphatidic acid metabolic process]
GO:0046474 [glycerophospholipid biosynthetic process]
GO:0048017 [inositol lipid-mediated signaling]
GO:0048471 [perinuclear region of cytoplasm]
GO:0048870 [cell motility]
GO:0050764 [regulation of phagocytosis]
GO:0060627 [regulation of vesicle-mediated transport]
GO:0070290 [N-acylphosphatidylethanolamine-specific phospholipase D activity]
Show all
1036 aa
119.7 kDa
No 0
PLD1-003
ENSP00000395556
ENST00000446289
H7C0L3 [Direct mapping]
Phospholipase D1
Show all
Predicted intracellular proteins
RAS pathway related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003824 [catalytic activity]
GO:0008152 [metabolic process]
Show all
234 aa
26.2 kDa
No 0
PLD1-010
ENSP00000400639
ENST00000418087
C9IY79 [Direct mapping]
Phospholipase D1
Show all
Predicted intracellular proteins
RAS pathway related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0035091 [phosphatidylinositol binding]
Show all
142 aa
16.6 kDa
No 0
PLD1-201
ENSP00000328422
ENST00000331659
Q8WYW5 [Direct mapping]
Phospholipase D1; Putative uncharacterized protein pp13439
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   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
RAS pathway related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
152 aa
16.5 kDa
No 0
PLD1-202
ENSP00000486967
ENST00000627725
F8WBV7 [Direct mapping]
Phospholipase D1
Show all
Predicted intracellular proteins
RAS pathway related proteins
Protein evidence (Ezkurdia et al 2014)
Show all
57 aa
6.5 kDa
No 0

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