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NPHP4

ANTIBODY INFORMATION
 
 

Antibody HPA065526

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA065526  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 13  

References

  

Proper citation

 Atlas Antibodies Cat#HPA065526, RRID:AB_2685505  

VALIDATION SUMMARY
 
 

Antibody HPA065526

  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA065526

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human fallopian tube shows strong membranous positivity in glandular cells.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA065526

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

 

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 157.6, 100  

Antibody dilution

 1:170  

PROTEIN ARRAY
 
 

Antibody HPA065526

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2050  

ANTIGEN INFORMATION
 
 

Antibody HPA065526

  

Antigen

 Recombinant protein fragment  

Length (aa)

 113  

Antigen sequence

 
SQALVLRSRLRLPEMVGHPAFAVIFQLEYVFSSPAGVDGNAASVTSLSNL
ACMHMVRWAVWNPLLEADSGRVTLPLQGGIQPNPSHCLVYKVPSASMSSE
EVKQVESGTLRFQ
 

Matching transcripts

 NPHP4-001 - ENSP00000367398 [100%]
NPHP4-201 - ENSP00000481831 [100%]
 

Other gene match

  
ANTIGEN VIEW
NPHP4-001
NPHP4-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.