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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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C3
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene namei

Gene name according to HGNC.

C3

Gene descriptioni

Gene description according to HGNC.

Complement component 3

Protein classi

Protein class the gene product belongs to according to selected gene lists. List of protein classes.

Candidate cardiovascular disease genes
Disease related genes
FDA approved drug targets
Plasma proteins
Predicted intracellular proteins
Predicted secreted proteins

Predicted localizationi

Protein localization predicted from several bioinformatic algorithms.

Read more

Intracellular,Secreted

Number of transcriptsi

Number of protein-coding transcribed from this gene as defined by Ensembl.

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA categoryi

RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue/cell line enriched, group enriched, tissue/cell line enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for The Cancer Genome Atlas (TCGA) data from cancer tissues and internally generated Human Protein Atlas (HPA) data from normal tissues and cell lines.

TCGA (cancer tissue):Expressed in all
HPA (cell line):Group enriched (BJ hTERT+, EFO-21, Hep G2, SiHa)
HPA (normal tissue):Expressed in all

Protein evidencei

Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.

Evidence at protein level

Protein expression
normal tissuei

A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.

"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.

Read more

Distinct positivity in plasma.

ANTIBODY IHC RELIABILITY

Data reliability
descriptioni

Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.

Secreted protein, tissue location of RNA and protein might differ and correlation is complex.

Reliability scorei

Reliability score (score description), divided into Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between the staining pattern of one antibody or several antibodies with RNA-seq data and available gene/protein characterization data.

Read more

Supported based on 3 antibodies.
HPA003563 , HPA020432 , CAB004209
SHOW MORE

PROGNOSTIC SUMMARYi

Kaplan-Meier plots for all cancers where high expression of this gene has significant (p<0.001) association with patient survival are shown in this summary. Whether the prognosis is favourable or unfavourable is indicated in brackets. Each Kaplan-Meier plot is clickable and redirects to a detailed page that includes individual expression and survival data for patients with the selected cancer.

Prognostic marker in renal cancer (unfavourable) and liver cancer (favourable).
Renal cancer p<0.001
Liver cancer p<0.001
Show all

RNA EXPRESSION OVERVIEWi

RNA expression overview shows RNA-seq data from The Cancer Genome Atlas (TCGA).

TCGA dataseti
RNA-seq data in 17 cancer types are reported as median FPKM (number Fragments Per Kilobase of exon per Million reads), generated by the The Cancer Genome Atlas (TCGA). RNA cancer tissue category is calculated based on mRNA expression levels across all 17 cancer tissues and include: cancer tissue enriched, cancer group enriched, cancer tissue enhanced, expressed in all, mixed and not detected. To access cancer specific RNA and prognostic data, click on the cancer name.

Read more
RNA cancer category: Expressed in all

PROTEIN EXPRESSIONi

Antibody staining in 20 different cancers is summarized by a selection of four standard cancer tissue samples representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional fifth image can be added as a complement. The assay and annotation is described here. Note that samples used for immunohistochemistry by the Human Protein Atlas do not correspond to samples in the TCGA dataset.

For histological reference, visit the histological dictionary.

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARYi

For each cancer, the fraction of samples with protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the left). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The mouse-over function displays additional data for the features in the staining summary view.

Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.

HPA003563
HPA020432
CAB004209

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summaryi

A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.

Moderate cytoplasmic, luminal membranous and extra cellular staining was observed in endometrial cancer as well as in cases of ovarian, urothelial, liver and pancreatic cancers. Other cancer tissues were mainly negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summaryi

A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.

A majority of malignant tissues displayed weak to moderate cytoplasmic staining. Most malignant lymphomas as well as several breast, cervical and skin cancers were negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summaryi

A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.

Distinct positivity was observed in tumor stroma. Most malignant cells displayed weak to moderate cytoplasmic immunoreactivity. Strong staining was found in a few ovarian-, endometrial-, testicular- and renal cancers.

GENE INFORMATIONi

Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.

Gene name

C3 (HGNC Symbol)

Synonyms

ARMD9, C3a, C3b, CPAMD1

Description

Complement component 3 (HGNC Symbol)

Entrez gene summary

Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form the mature protein, which is then further processed to generate numerous peptide products. The C3a peptide, also known as the C3a anaphylatoxin, modulates inflammation and possesses antimicrobial activity. Mutations in this gene are associated with atypical hemolytic uremic syndrome and age-related macular degeneration in human patients. [provided by RefSeq, Nov 2015]

Chromosome

19

Cytoband

p13.3

Chromosome location (bp)

6677704 - 6730562

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000125730 (version 83.38)

Entrez gene

718

UniProt

P01024 (UniProt - Evidence at protein level)

neXtProt

NX_P01024

Antibodypedia

C3 antibodies

PROTEIN BROWSERi

The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.

At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.

Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).

If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.

Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.

C3-001
C3-003
C3-014
C3-015
C3-018

PROTEIN INFORMATIONi

The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.

The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.

The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.

The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

C3-001
ENSP00000245907
ENST00000245907
P01024 [Direct mapping]
Complement C3 Complement C3 beta chain C3-beta-c Complement C3 alpha chain C3a anaphylatoxin Acylation stimulating protein Complement C3b alpha' chain Complement C3c alpha' chain fragment 1 Complement C3dg fragment Complement C3g fragment Complement C3d fragment Complement C3f fragment Complement C3c alpha' chain fragment 2
V9HWA9 [Target identity:100%; Query identity:100%]
Epididymis secretory sperm binding protein Li 62p
Show all
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Candidate cardiovascular disease genes
Disease related genes
FDA approved drug targets
   Biotech drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001798 [positive regulation of type IIa hypersensitivity]
GO:0001934 [positive regulation of protein phosphorylation]
GO:0001970 [positive regulation of activation of membrane attack complex]
GO:0002507 [tolerance induction]
GO:0004866 [endopeptidase inhibitor activity]
GO:0005102 [receptor binding]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0005886 [plasma membrane]
GO:0006631 [fatty acid metabolic process]
GO:0006954 [inflammatory response]
GO:0006955 [immune response]
GO:0006956 [complement activation]
GO:0006957 [complement activation, alternative pathway]
GO:0006958 [complement activation, classical pathway]
GO:0007165 [signal transduction]
GO:0007186 [G-protein coupled receptor signaling pathway]
GO:0007596 [blood coagulation]
GO:0008289 [lipid binding]
GO:0010575 [positive regulation of vascular endothelial growth factor production]
GO:0010828 [positive regulation of glucose transport]
GO:0010866 [regulation of triglyceride biosynthetic process]
GO:0010884 [positive regulation of lipid storage]
GO:0010951 [negative regulation of endopeptidase activity]
GO:0030449 [regulation of complement activation]
GO:0031715 [C5L2 anaphylatoxin chemotactic receptor binding]
GO:0032026 [response to magnesium ion]
GO:0032355 [response to estradiol]
GO:0032570 [response to progesterone]
GO:0043627 [response to estrogen]
GO:0045087 [innate immune response]
GO:0045745 [positive regulation of G-protein coupled receptor protein signaling pathway]
GO:0045766 [positive regulation of angiogenesis]
GO:0048037 [cofactor binding]
GO:0048639 [positive regulation of developmental growth]
GO:0050766 [positive regulation of phagocytosis]
GO:0050776 [regulation of immune response]
GO:0051384 [response to glucocorticoid]
GO:0070062 [extracellular exosome]
GO:0070374 [positive regulation of ERK1 and ERK2 cascade]
GO:0072562 [blood microparticle]
GO:2000427 [positive regulation of apoptotic cell clearance]
Show all
1663 aa
187.1 kDa
Yes 0
C3-003
ENSP00000472044
ENST00000600744
M0R1Q1 [Direct mapping]
Complement C3
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
103 aa
11.2 kDa
No 0
C3-014
ENSP00000469482
ENST00000602229
M0QXZ3 [Direct mapping]
Complement C3
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
Show all
100 aa
11.6 kDa
No 0
C3-015
ENSP00000471384
ENST00000601008
M0R0Q9 [Direct mapping]
Complement C3
Show all
   Phobius predicted secreted proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005615 [extracellular space]
Show all
101 aa
11.2 kDa
No 0
C3-018
ENSP00000469744
ENST00000596548
M0QYC8 [Direct mapping]
Complement C3
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005576 [extracellular region]
Show all
192 aa
21.7 kDa
No 0

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The Human Protein Atlas project is funded
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