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TOP2A

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GENERAL INFORMATION

Gene name

 TOP2A

Gene description

 Topoisomerase (DNA) II alpha

Protein class

 Cancer-related genes
Enzymes
FDA approved drug targets
Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 3
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HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the nucleus. In addition localized to the nucleoli.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Nucleus (validated)

Additional location

 In addition localized to the Nucleoli (approved)

Single-cell variation

 Single-cell variation in protein expression observed.

Cell cycle dependency

 Cell cycle dependent expression determined by custom assay

DATA RELIABILITY

Reliability score

 Validated based on 2 antibodies.
CAB002448 , HPA006458
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ASSAYS
RNA expression
Human cells
Custom data

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the nucleus. In addition localized to the nucleoli.

Main location

 Nucleus (validated)

Additional location

 Nucleoli (approved)

 

 

 
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Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
CAB002448 A-431 Nucleoplasm Intensity
CAB002448 U-2 OS Nucleoplasm Intensity
CAB002448 U-251 MG Nucleoplasm Intensity
HPA006458 A-431 Nucleus Intensity
Nucleoli Intensity
HPA006458 U-2 OS Nucleus Intensity Intensity
HPA006458 U-251 MG Nucleus Intensity

CUSTOM DATA

 

 

 
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Thumbnail

Antibody

Cell line

Assay

Description
HPA006458 U-2 OS Image Staining of U-2 OS FUCCI cells, to characterize the cell cycle dependency of the protein expression pattern. The FUCCI cells, Fluorescence Ubiquitination Cell Cycle Indicator, are cells tagged with different fluorescent proteins fused to two different cell cycle regulators Cdt1 (expressed in G1 phase) and Geminin (expressed in S and G2 phases) that allows cell cycle monitoring. When both proteins are present, the overlay of the images appear in yellow marking the G1/S transition. TOP2A is mostly expressed during S and/or G2, and during Mitosis.

CELL CYCLE

Summary

 Expression has cell cycle dependency according to custom assay

Intensity variation

 Nucleoli, Nucleus

GENE INFORMATION

Gene name

 TOP2A (HGNC Symbol)

Synonyms

 TOP2

Description

 Topoisomerase (DNA) II alpha (HGNC Symbol)

Entrez gene summary

 This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]

Chromosome

 17

Cytoband

 q21.2

Chromosome location (bp)

 40388516 - 40417950

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000131747 (version 83.38)

Entrez gene

 7153

UniProt

 P11388 (UniProt - Evidence at protein level)

neXtProt

 NX_P11388

Antibodypedia

 TOP2A antibodies

PROTEIN BROWSER
TOP2A-001
TOP2A-003
TOP2A-006

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
TOP2A-001
ENSP00000411532
ENST00000423485
P11388 [Direct mapping]
DNA topoisomerase 2-alpha
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Enzymes
   ENZYME proteins
   Isomerase
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Candidate cancer biomarkers
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000228 [nuclear chromosome]
GO:0000278 [mitotic cell cycle]
GO:0000287 [magnesium ion binding]
GO:0000712 [resolution of meiotic recombination intermediates]
GO:0000793 [condensed chromosome]
GO:0000819 [sister chromatid segregation]
GO:0002244 [hematopoietic progenitor cell differentiation]
GO:0003677 [DNA binding]
GO:0003682 [chromatin binding]
GO:0003918 [DNA topoisomerase type II (ATP-hydrolyzing) activity]
GO:0005080 [protein kinase C binding]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0005814 [centriole]
GO:0006265 [DNA topological change]
GO:0006266 [DNA ligation]
GO:0006268 [DNA unwinding involved in DNA replication]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007059 [chromosome segregation]
GO:0008022 [protein C-terminus binding]
GO:0008094 [DNA-dependent ATPase activity]
GO:0008144 [drug binding]
GO:0008301 [DNA binding, bending]
GO:0009330 [DNA topoisomerase complex (ATP-hydrolyzing)]
GO:0019035 [viral integration complex]
GO:0019899 [enzyme binding]
GO:0030261 [chromosome condensation]
GO:0030263 [apoptotic chromosome condensation]
GO:0040016 [embryonic cleavage]
GO:0042752 [regulation of circadian rhythm]
GO:0042803 [protein homodimerization activity]
GO:0042826 [histone deacetylase binding]
GO:0043065 [positive regulation of apoptotic process]
GO:0043130 [ubiquitin binding]
GO:0043234 [protein complex]
GO:0044774 [mitotic DNA integrity checkpoint]
GO:0044822 [poly(A) RNA binding]
GO:0045070 [positive regulation of viral genome replication]
GO:0045870 [positive regulation of single stranded viral RNA replication via double stranded DNA intermediate]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0046982 [protein heterodimerization activity]
GO:0048511 [rhythmic process]
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 1531 aa
174.4 kDa 		No 0
TOP2A-003
ENSP00000462908
ENST00000581055
J3KTB7 [Direct mapping]
DNA topoisomerase 2-alpha
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000712 [resolution of meiotic recombination intermediates]
GO:0000819 [sister chromatid segregation]
GO:0003677 [DNA binding]
GO:0003918 [DNA topoisomerase type II (ATP-hydrolyzing) activity]
GO:0005524 [ATP binding]
GO:0006265 [DNA topological change]
GO:0006268 [DNA unwinding involved in DNA replication]
GO:0044774 [mitotic DNA integrity checkpoint]
Show all
 147 aa
17.1 kDa 		No 0
TOP2A-006
ENSP00000464055
ENST00000577541
J3QR57 [Direct mapping]
DNA topoisomerase 2-alpha
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000712 [resolution of meiotic recombination intermediates]
GO:0000819 [sister chromatid segregation]
GO:0003677 [DNA binding]
GO:0003918 [DNA topoisomerase type II (ATP-hydrolyzing) activity]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0006268 [DNA unwinding involved in DNA replication]
GO:0044774 [mitotic DNA integrity checkpoint]
Show all
 27 aa
3.1 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.