Methods

Antibodies are used in a variety of research applications and techniques, such as Western Blot, Enzyme-Linked Immunosorbent Assay, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, and more. The different conditions in each application and the complexity of biological samples ask for great functionality from the antibodies.

The following pages describe some of the most common techniques.

Antibodypedia

Enzyme-linked immunosorbent assay

Flow cytometry

Immunocytochemistry

Immunoelectron microscopy

Immunohistochemistry

Immunoprecipitation

Immunoproteomics

Proximity ligation assay

Western blot

References and Links

• An overview of the immune system:
  The Immune System: In Defence of our Lives. (n.d.). Retrieved February 20, 2014, from http://www.nobelprize.org/educational/medicine/immuneresponses/overview/index.html



• About the Nobel Prize in Physiology or Medicine 1901:
  The Nobel Prize in Physiology or Medicine 1901. (n.d.). Retrieved February 19, 2014, from http://www.nobelprize.org/nobel_prizes/medicine/laureates/1901/


• A summary of the immune system and the breakthroughs for which the Nobel Prize has been awarded:
  http://www.nobelprize.org/nobel_prizes/medicine/immune_responses.html


• Relevant Wikipedia pages:
  http://en.wikipedia.org/wiki/Antibodies
  http://en.wikipedia.org/wiki/Polyclonal_antibodies
  http://en.wikipedia.org/wiki/Monoclonal_antibody


• Antibodypedia –- An open-access database of publicly available antibodies and their usefulness in various applications:
  http://www.antibodypedia.com