Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of cytokinetic bridge in human cell line MCF-7 (HPA046743)
Scale bar represents 10µm
The cytokinetic bridge is a structure present during the last stage of cell division, the cytokinesis. It is composed of tubulin filaments protruding from the cleavage furrow. The width and length of the cytokinetic bridge is dependent on the progression of the cytokinesis. In the latest stage of cytokinesis the bridge is cleaved and the cells are separated.
The midbody can be referred to as the tip of the cytokinetic bridge and consists of tightly bundled microtubules, which embed the midbody ring. It is responsible for many cytokinesis-related processes including cytoskeleton remodeling or degradation of cell cycle regulators.
The midbody ring is a circular structure that mainly consists of actin filaments. It is embedded in the space between the midbodies of the daughter cells. It is mainly responsible for the cleavage of the cytokinetic bridge, thus finishing the process of cytokinesis.
The cytokinetic bridge staining appears as a staining of the microtubule filaments protruding from the two daughter cells. The midbody staining is characterized by an exclusive staining of the tips of the microtubule filaments at the contact surface whereas the midbody ring appears as a circular structure in the space between the two midbodies.