We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.
Antibody dilution
1:172
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA043018
Standard validation
Approved
Figure description
Immunohistochemical staining of human breast shows strong nucleolar positivity in glandular cells.
Expression
RNA: detected in 34 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043018
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411419)
Target mass (kDa)
30.3, 19.6, 17.8, 17
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA043018
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
