We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Fields »
 

Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
ABOUT
HELP
BLOG
  • THE HUMAN PROTEOME
  • THE HUMAN TISSUES
  • THE HUMAN CELL
  • HUMAN PATHOLOGY
  • PROTEIN CLASSES
  • PROTEIN EVIDENCE
    • NEWS
    • BLOG
    • EVENTS
    • PRESS ROOM
    • LEARN
    • DICTIONARY
    • METHODS
    • CELL LINES
  • THE PROJECT
  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • PUBLICATION DATA
  • ANTIBODY SUBMISSION
  • ANTIBODY AVAILABILITY
  • LINKS
  • CONTACT
  • TECHNICAL DATA
  • ANTIBODY VALIDATION
  • ASSAYS & ANNOTATION
  • DISCLAIMER
  • DOWNLOADABLE DATA
  • HELP & FAQ
  • LICENCE & CITATION
  • PRIVACY STATEMENT
  • RELEASE HISTORY
CDC27
  • premium

    TISSUE

  • premium

    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

CDC27

Gene description

Cell division cycle 27

Protein class

Cancer-related genes
Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

5
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining consistent with RNA expression data.

Reliability score

Supported based on 2 antibodies.
HPA028129 , CAB016315
SHOW MORE

PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable), colorectal cancer (favourable), liver cancer (unfavourable) and lung cancer (unfavourable).
Renal cancer p<0.001
Colorectal cancer p<0.001
Liver cancer p<0.001
Lung cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA028129
CAB016315

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most malignancies displayed moderate to strong nuclear staining combined with cases of cytoplasmic positivity. Non-hodgkin's lymphomas, prostate and renal cell carcinomas were weakly stained or negative in several cases.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Malignancies generally displayed moderate to strong nuclear positivity. A majority of adenocarcinomas in lung, hepatocellular, prostate and renal cancers were weakly stained or negative.

GENE INFORMATION

Gene name

CDC27 (HGNC Symbol)

Synonyms

ANAPC3, APC3, D0S1430E, D17S978E, NUC2

Description

Cell division cycle 27 (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014]

Chromosome

17

Cytoband

q21.32

Chromosome location (bp)

47117703 - 47189422

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000004897 (version 83.38)

Entrez gene

996

UniProt

P30260 (UniProt - Evidence at protein level)

neXtProt

NX_P30260

Antibodypedia

CDC27 antibodies

PROTEIN BROWSER

CDC27-001
CDC27-002
CDC27-005
CDC27-012
CDC27-014

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

CDC27-001
ENSP00000066544
ENST00000066544
P30260 [Direct mapping]
Cell division cycle protein 27 homolog
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000278 [mitotic cell cycle]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005680 [anaphase-promoting complex]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005819 [spindle]
GO:0005829 [cytosol]
GO:0005876 [spindle microtubule]
GO:0007091 [metaphase/anaphase transition of mitotic cell cycle]
GO:0007094 [mitotic spindle assembly checkpoint]
GO:0008283 [cell proliferation]
GO:0019903 [protein phosphatase binding]
GO:0031145 [anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process]
GO:0051436 [negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0051437 [positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition]
GO:0051439 [regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0070979 [protein K11-linked ubiquitination]
Show all
824 aa
91.9 kDa
No 0
CDC27-002
ENSP00000434614
ENST00000531206
P30260 [Direct mapping]
Cell division cycle protein 27 homolog
Show all
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000278 [mitotic cell cycle]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005680 [anaphase-promoting complex]
GO:0005737 [cytoplasm]
GO:0005813 [centrosome]
GO:0005819 [spindle]
GO:0005829 [cytosol]
GO:0005876 [spindle microtubule]
GO:0007091 [metaphase/anaphase transition of mitotic cell cycle]
GO:0007094 [mitotic spindle assembly checkpoint]
GO:0008283 [cell proliferation]
GO:0019903 [protein phosphatase binding]
GO:0031145 [anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process]
GO:0051436 [negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0051437 [positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition]
GO:0051439 [regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle]
GO:0070979 [protein K11-linked ubiquitination]
Show all
830 aa
92.6 kDa
No 0
CDC27-005
ENSP00000437339
ENST00000527547
G5EA36 [Direct mapping]
Cell division cycle 27, isoform CRA_c; Cell division cycle protein 27 homolog
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
GO:0005654 [nucleoplasm]
GO:0051301 [cell division]
Show all
823 aa
91.7 kDa
No 0
CDC27-012
ENSP00000460454
ENST00000575483
I3L3H6 [Direct mapping]
Cell division cycle protein 27 homolog
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005654 [nucleoplasm]
Show all
233 aa
25.9 kDa
No 0
CDC27-014
ENSP00000460279
ENST00000571643
I3L394 [Direct mapping]
Cell division cycle protein 27 homolog
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
Show all
163 aa
18.8 kDa
No 0

Contact

  • BLOG
  • PRESS ROOM
  • contact@proteinatlas.org

The Project

  • INTRODUCTION
  • ORGANIZATION
  • PUBLICATIONS
  • DOWNLOADABLE DATA

The Human Protein Atlas

  • ANTIBODY AVAILABILITY
  • LICENCE & CITATION
  • HELP & FAQ
The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.