Fields »
ABOUT
HELP
BLOG
SLC4A1

ANTIBODY INFORMATION
 
 

Antibody HPA015584

  

Antibody HPA063911

  

Antibody CAB034438

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA015584   HPA063911   sc-133190  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 4   14   14  

References

      

Proper citation

 Atlas Antibodies Cat#HPA015584, RRID:AB_1844824   Atlas Antibodies Cat#HPA063911, RRID:AB_2685151   Santa Cruz Biotechnology Cat#sc-133190, RRID:AB_2123479  

VALIDATION SUMMARY
 
 

Antibody HPA015584

  

Antibody HPA063911

  

Antibody CAB034438

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain    
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015584

  

Antibody HPA063911

  

Antibody CAB034438

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human colon shows distinct positivity in erythrocytes.   Immunohistochemical staining of human spleen shows strong positivity in erythrocytes.   Immunohistochemical staining of human lung shows distinct positivity in erythrocytes.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 6 cell types		   RNA: detected in 6 tissues
Protein: detected in 0 cell types		   RNA: detected in 6 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:2000   1:60000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015584

  

Antibody HPA063911

  

Antibody CAB034438

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		    

     

Figure description

      

Target mass (kDa)

 101.8, 60.8   101.8, 60.8    

Antibody dilution

 1:250   1:270    

PROTEIN ARRAY
 
 

Antibody HPA015584

  

Antibody HPA063911

  

Antibody CAB034438

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3250    

ANTIGEN INFORMATION
 
 

Antibody HPA015584

  

Antibody HPA063911

  

Antibody CAB034438

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 104   98    

Antigen sequence

 
LLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLFCEQGDGG
TEGHSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEAAELEAVE
LPVP
  
DDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTSHPGTH
KVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLSHLT
  

											 
																				

																		

										
											
Matching transcripts

												
																					SLC4A1-001 - ENSP00000262418 [100%] 
SLC4A1-201 - ENSP00000382190 [98%] 

											 
																						SLC4A1-001 - ENSP00000262418 [100%] 
SLC4A1-201 - ENSP00000382190 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SLC4A1-001
SLC4A1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.