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CREBBP

ANTIBODY INFORMATION
 
 

Antibody HPA055861

  

Antibody CAB004212

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA055861   sc-7300  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 12   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA055861, RRID:AB_2682948   Santa Cruz Biotechnology Cat#sc-7300, RRID:AB_626817  

VALIDATION SUMMARY
 
 

Antibody HPA055861

  

Antibody CAB004212

  
Immunocytochemistry


Validated
   Supported  
Immunohistochemistry


Validated
   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055861

  

Antibody CAB004212

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

   

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:93    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear bodies.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:93   1:50  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055861

  

Antibody CAB004212

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

   

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 73 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:150   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA055861

  

Antibody CAB004212

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

   

Figure description

    

Target mass (kDa)

 265.4, 261, 122.4   265.4, 261, 122.4, 27.2, 22.5, 19  

Antibody dilution

 1:460   1:500  

PROTEIN ARRAY
 
 

Antibody HPA055861

  

Antibody CAB004212

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3700    

ANTIGEN INFORMATION
 
 

Antibody HPA055861

  

Antibody CAB004212

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 73    

Antigen sequence

 
VASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQ
GASQVKEETDIAEQKSEPMEVDE
  

											 
																				

																		

										
											
Matching transcripts

												
																					CREBBP-001 - ENSP00000262367 [100%] 
CREBBP-003 - ENSP00000371502 [100%] 
CREBBP-008 - ENSP00000461002 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CREBBP-001
CREBBP-003
CREBBP-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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