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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria.
Antibody dilution
1:47
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA032005
Antibody HPA032006
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human pancreas shows strong granular cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 71 cell types
RNA: detected in 37 tissues Protein: detected in 81 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA032005
Antibody HPA032006
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420021)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY420021)
Target mass (kDa)
48.9, 19.7
48.9, 19.7
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA032005
Antibody HPA032006
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
